电化学直接检测 APOBEC3G 催化的 DNA 脱氨酶活性。
Electrochemical direct detection of DNA deamination catalyzed by APOBEC3G.
机构信息
Graduate School of Pharmaceutical Sciences, University of Toyama, Sugitani 2630, Toyama 930-0194, Japan.
出版信息
Chem Commun (Camb). 2012 Dec 25;48(99):12115-7. doi: 10.1039/c2cc36779c. Epub 2012 Nov 12.
Abstract
APOBEC3G catalyzes deamination of cytosines in HIV-1 genome, and restricts the HIV-1 infection. Here, we propose a picomole-scale assay for the detection of DNA deamination catalyzed by APOBEC3G. Our results show the suitability of the developed method for a time course analysis of enzyme-catalyzed DNA modifications.
摘要
APOBEC3G 可催化 HIV-1 基因组中的胞嘧啶脱氨酶，从而限制 HIV-1 的感染。在此，我们提出了一种皮摩尔级别的检测 APOBEC3G 催化的 DNA 脱氨的方法。我们的结果表明，该方法适用于酶催化的 DNA 修饰的时间过程分析。
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