Cléry Antoine, Schubert Mario, Allain Frédéric H-T
Institute for Molecular Biology and Biophysics ETH Zürich CH-8093 Zürich, Switzerland.
Chimia (Aarau). 2012;66(10):741-6. doi: 10.2533/chimia.2012.741.
Structural biology plays a key role in understanding how networks of protein interactions with their partners are organized at the atomic level. In this review, we show that NMR is a very efficient method to solve 3D structures of protein - RNA and protein-carbohydrate complexes of high quality. We explain the importance of studying such interactions and describe the main steps that are required to determine structures of these types of complexes by NMR. Finally, we show that X-ray crystallography and NMR are complementary methods and briefly report on advantages and disadvantages of each approach.
结构生物学在理解蛋白质与其伙伴的相互作用网络如何在原子水平上组织方面发挥着关键作用。在本综述中，我们表明核磁共振（NMR）是解析高质量蛋白质-RNA和蛋白质-碳水化合物复合物三维结构的一种非常有效的方法。我们解释了研究此类相互作用的重要性，并描述了通过核磁共振确定这些类型复合物结构所需的主要步骤。最后，我们表明X射线晶体学和核磁共振是互补的方法，并简要报告了每种方法的优缺点。
