Departamento de Análises Clínicas e Toxicológicas, Faculdade de Medicina, Universidade Federal do Ceará, Fortaleza, CE, Brasil.
Mem Inst Oswaldo Cruz. 2012 Nov;107(7):873-6. doi: 10.1590/s0074-02762012000700006.
Laboratory diagnosis of intestinal schistosomiasis mansoni can be accomplished through various methods of stool examination to detect parasites, ranging from the most classic tests (Kato-Katz) to several methods that are still undergoing validation. This study was conducted to assess two new parasite identification methods for diagnosing schistosomiasis mansoni in residents of a low endemic area in the municipality of Maranguape, in the state of Ceará, Brazil using the Kato-Katz method as a reference and serology (enzyme-linked immunosorbent assay) for the screening of patients. The Kato-Katz, the saline gradient method and the Helmintex(®) method parasite identification methods were employed only in subjects who exhibited positive serologic tests. The test results were then analysed and treatment of positive individuals was subsequently performed. After comparing the test results, we observed that the saline gradient method and the Helmintex(®) method were more effective in diagnosing schistosomiasis mansoni in the study area compared with the Kato-Katz method.
曼氏血吸虫病的实验室诊断可通过粪便检查来检测寄生虫的各种方法来完成,从最经典的检测方法(加藤厚涂片法)到几种仍在验证中的方法。本研究旨在评估两种新的寄生虫鉴定方法,用于巴西塞阿拉州马拉兰瓜佩市低流行地区居民的曼氏血吸虫病诊断,加藤厚涂片法作为参考,酶联免疫吸附试验(ELISA)用于筛查患者。加藤厚涂片法、盐水梯度法和 Helmintex(®)方法寄生虫鉴定法仅用于血清学检测呈阳性的受试者。然后对检测结果进行分析,并对阳性个体进行治疗。比较检测结果后,我们发现与加藤厚涂片法相比,在研究区域,盐水梯度法和 Helmintex(®)法在诊断曼氏血吸虫病方面更有效。