抗-HEV IgG 检测方法的检测性能特征强烈影响戊型肝炎血清流行率的估计。

Test performance characteristics of Anti-HEV IgG assays strongly influence hepatitis E seroprevalence estimates.

机构信息

Institute of Medical Microbiology and Hygiene, University of Regensburg, Regensburg, Germany.

出版信息

J Infect Dis. 2013 Feb 1;207(3):497-500. doi: 10.1093/infdis/jis688. Epub 2012 Nov 12.

Abstract

Hepatitis E virus (HEV) seroprevalences of 0.3%-53% were reported from industrialized countries. Because these estimates may be influenced by detection assays, this study compares 3 frequently used tests for HEV detection: the MP Diagnostics HEV immunoglobulin G (IgG) enzyme-linked immunosorbent assay (ELISA), the Axiom Diagnostics HEV IgG enzyme immunoassay (EIA), and the Mikrogen recomLine HEV IgG assay. Sera from 200 healthy healthcare workers and 30 individuals with acute HEV infection were analyzed. Among the healthy individuals, HEV IgG was found in 4.5% by the MP Diagnostics assay, in 29.5% by the Axiom Diagnostics assay, and in 18% by the Mikrogen assay. Among individuals with acute HEV infection, positive results were obtained for 83.3%, 100%, and 96.7%, respectively. Thus, the 3 assays show clear differences in diagnostic sensitivity.

摘要

在工业化国家，报道的戊型肝炎病毒 (HEV) 血清流行率为 0.3%-53%。由于这些估计可能受到检测方法的影响，本研究比较了 3 种常用于检测 HEV 的检测方法：MP Diagnostics 的戊型肝炎病毒 IgG 酶联免疫吸附测定 (ELISA)、Axiom Diagnostics 的戊型肝炎病毒 IgG 酶免疫测定 (EIA) 和 Mikrogen 的 recomLine HEV IgG 测定。分析了 200 名健康医护人员和 30 名急性 HEV 感染者的血清。在健康个体中，MP Diagnostics 检测法发现 4.5%的人存在 HEV IgG，Axiom Diagnostics 检测法发现 29.5%的人存在 HEV IgG，Mikrogen 检测法发现 18%的人存在 HEV IgG。在急性 HEV 感染者中，分别有 83.3%、100%和 96.7%的人获得阳性结果。因此，这 3 种检测方法在诊断灵敏度上存在明显差异。