四种戊型肝炎抗体检测方法的性能比较

Performance Comparison of Four Hepatitis E Antibodies Detection Methods.

作者信息

Muñoz-Chimeno Milagros, Díaz-Sánchez Nazaret, Morago Lucía, Rodríguez-Paredes Vanessa, Barturen Silvia, Rodríguez-Recio Álvaro, García-Lugo Maira Alejandra, Zamora Maria Isabel, Mateo María, Sánchez-Martínez Mónica, Avellón Ana

机构信息

Hepatitis Unit, National Center of Microbiology, Instituto de Salud Carlos III, 28220 Madrid, Spain.

Servicio de Microbiología, Hospital Central de la Defensa, 28047 Madrid, Spain.

出版信息

Microorganisms. 2024 Sep 11;12(9):1875. doi: 10.3390/microorganisms12091875.

DOI:10.3390/microorganisms12091875

PMID:39338549

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11434459/

Abstract

HEV antibody detection constitutes the main screening test for HEV infection. The aim of this study is to compare the sensitivity and specificity of four techniques: LIAISON MUREX DiaSorin anti-HEV IgG and anti-HEV IgM assays, Hepatitis E VIRCLIA IgM and IgG monotests, WANTAI HEV-IgM and IgG ELISA and VIDAS anti-HEV IgM and IgG tests in five panels of samples configurated according to the immunoblot (RecomLine, Mikrogen, Neuss, Germany). Anti-HEV IgM sensitivity in the acute phase was 100% in all techniques, while sensitivity, including the immediate convalescence phase, was 96.74% for LIAISON, 83.14% for VIRCLIA, 84.78% for WANTAI and 88.04% for VIDAS. Anti-HEV IgM specificity was 100% for both LIAISON and VIRCLIA. Anti-HEV IgM WANTAI agreed with VIRCLIA with a good Kappa coefficient (κ = 0.71). Anti-HEV IgG post-infection sensitivity was 100% for LIAISON, VIDAS and VIRCLIA and 99% for WANTAI. Anti-HEV IgG specificity reached 97.17% for LIAISON and 88.68% for VIRCLIA. Our results demonstrated a better capacity of LIAISON MUREX anti-HEV IgM than that of competitors for detecting acute infections as well as accurate anti-HEV IgG results and in how to resolve them.

摘要

戊型肝炎病毒（HEV）抗体检测是HEV感染的主要筛查试验。本研究旨在比较四种技术的敏感性和特异性：LIAISON MUREX DiaSorin抗HEV IgG和抗HEV IgM检测、戊型肝炎病毒VIRCLIA IgM和IgG单项检测、万泰HEV-IgM和IgG酶联免疫吸附测定（ELISA）以及VIDAS抗HEV IgM和IgG检测，这四种技术针对按照免疫印迹法（德国诺伊斯市Mikrogen公司的RecomLine）配置的五组样本进行检测。在急性期，所有技术检测抗HEV IgM的敏感性均为100%，而在包括恢复期早期阶段在内的整个过程中，LIAISON检测的敏感性为96.74%，VIRCLIA为83.14%，万泰为84.78%，VIDAS为88.04%。LIAISON和VIRCLIA检测抗HEV IgM的特异性均为100%。万泰抗HEV IgM与VIRCLIA的检测结果一致性良好，卡帕系数（κ）为0.71。感染后抗HEV IgG的敏感性，LIAISON、VIDAS和VIRCLIA均为100%，万泰为99%。LIAISON检测抗HEV IgG的特异性达到97.17%，VIRCLIA为88.68%。我们的结果表明，在检测急性感染方面，LIAISON MUREX抗HEV IgM的能力优于其他同类产品，并且其抗HEV IgG检测结果准确。