Department of Health, Exercise and Sports Science, University of New Mexico, University Blvd, Albuquerque, NM, 87131, USA.
Nutr Metab (Lond). 2012 Nov 13;9(1):101. doi: 10.1186/1743-7075-9-101.
Obesity is a common pathology with increasing incidence, and is associated with increased mortality and healthcare costs. Several treatment options for obesity are currently available ranging from behavioral modifications to pharmaceutical agents. Many popular dietary supplements claim to enhance weight loss by acting as metabolic stimulators, however direct tests of their effect on metabolism have not been performed.
This work identified the effects popular dietary supplements on metabolic rate and mitochondrial biosynthesis in human skeletal muscle cells.
Human rhabdomyosarcoma cells were treated with popular dietary supplements at varied doses for 24 hours. Peroxisome proliferator-activated receptor coactivator 1 alpha (PGC-1α), an important stimulator of mitochondrial biosynthesis, was quantified using quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). Mitochondrial content was measured using flow cytometry confirmed with confocal microscopy. Glycolytic metabolism was quantified by measuring extracellular acidification rate (ECAR) and oxidative metabolism was quantified by measuring oxygen consumption rate (OCR). Total relative metabolism was quantified using WST-1 end point assay.
Treatment of human rhabdomyosarcoma cells with dietary supplements OxyElite Pro (OEP) or Cellucore HD (CHD) induced PGC-1α leading to significantly increased mitochondrial content. Glycolytic and oxidative capacities were also significantly increased following treatment with OEP or CHD.
This is the first work to identify metabolic adaptations in muscle cells following treatment with popular dietary supplements including enhanced mitochondrial biosynthesis, and glycolytic, oxidative and total metabolism.
肥胖是一种常见的病理，发病率不断上升，与死亡率增加和医疗保健成本增加有关。目前有多种治疗肥胖的方法，从行为改变到药物治疗都有。许多流行的膳食补充剂声称通过作为代谢刺激物来促进体重减轻，但尚未对其对代谢的影响进行直接测试。
本研究旨在确定流行的膳食补充剂对人骨骼肌细胞代谢率和线粒体生物合成的影响。
用人横纹肌肉瘤细胞用不同剂量的流行膳食补充剂处理 24 小时。过氧化物酶体增殖物激活受体共激活因子 1α（PGC-1α）是线粒体生物合成的重要刺激物，使用定量逆转录聚合酶链反应（qRT-PCR）进行定量。使用流式细胞术测量线粒体含量，并通过共聚焦显微镜进行验证。通过测量细胞外酸化率（ECAR）来量化糖酵解代谢，通过测量耗氧量（OCR）来量化氧化代谢。使用 WST-1 终点测定法来量化总相对代谢。
用膳食补充剂 OxyElite Pro（OEP）或 Cellucore HD（CHD）处理人横纹肌肉瘤细胞会诱导 PGC-1α，从而导致线粒体含量显著增加。用 OEP 或 CHD 处理后，糖酵解和氧化能力也显著增加。
这是首次确定流行膳食补充剂处理后肌肉细胞中的代谢适应性，包括增强的线粒体生物合成以及糖酵解、氧化和总代谢。
