Laboratory for Infectious Diseases and Screening; National Institute of Public Health and the Environment; Bilthoven, the Netherlands; Department of Pathology; VU University Medical Centre; Amsterdam, the Netherlands.
Hum Vaccin Immunother. 2013 Feb;9(2):314-21. doi: 10.4161/hv.22693. Epub 2012 Nov 13.
The bivalent HPV16/18 vaccine induces high antibody concentrations in serum while data about antibody responses in the cervix are limited. In this study, we investigated pre- and post-vaccination antibody responses against seven high-risk HPV types by detection of IgG and IgA HPV-specific antibodies in cervical secretion samples (CVS) and serum. From an HPV vaccine monitoring study CVS and serum samples were available (pre-vaccination (n = 297), one year (n = 211) and two years (n = 141) post-dose-one vaccination) from girls aged 14-16 y. The girls were vaccinated with the bivalent HPV vaccine at months 0, 1 and 6. CVS was self-sampled using a tampon. Samples were tested for HPV-specific antibodies (HPV16/18/31/33/45/52/58) by a VLP-based multiplex immunoassay. Post-vaccination, IgG and IgA antibody levels for HPV16/18 were detectable in CVS and amounted to 2% and 1% of the IgG and IgA antibody levels observed in serum, respectively. The antibody levels remained constant between one and two years after vaccination. The correlation between CVS and serum was similar for IgG and IgA vaccine-derived antibody levels for HPV16 (rs = 0.58, rs = 0.54) and HPV18 (rs = 0.50, rs = 0.55). Vaccine-derived IgG antibody levels against cross-reactive HPV types in CVS and in serum were highest for HPV45. No IgA cross-reactive antibody responses could be detected in CVS. Post-vaccination, HPV16/18 IgG and IgA antibodies are not only detectable in serum but also in CVS. The correlation of HPV16/18 IgG antibody levels between serum and CVS suggests that vaccine induced HPV antibodies transudate and/or exudate from the systemic circulation to the cervical mucosa to provide protection against HPV infections.
二价 HPV16/18 疫苗可在血清中诱导高浓度抗体，而有关宫颈抗体反应的数据有限。在这项研究中，我们通过检测宫颈分泌物样本（CVS）和血清中的 IgG 和 IgA 型 HPV 特异性抗体，研究了七型高危 HPV 疫苗接种前后的抗体反应。在 HPV 疫苗监测研究中，从 14-16 岁的女孩中获得了 CVS 和血清样本（疫苗接种前（n=297）、接种后一年（n=211）和两年（n=141））。女孩们在 0、1 和 6 个月时接种了二价 HPV 疫苗。使用棉塞自行采集 CVS。通过基于 VLPs 的多重免疫测定法检测 HPV 特异性抗体（HPV16/18/31/33/45/52/58）。接种疫苗后，可在 CVS 中检测到 HPV16/18 的 IgG 和 IgA 抗体，分别占血清中 IgG 和 IgA 抗体水平的 2%和 1%。接种疫苗后 1 至 2 年内，抗体水平保持不变。CVS 和血清之间的相关性对于 HPV16（rs=0.58，rs=0.54）和 HPV18（rs=0.50，rs=0.55）的疫苗衍生 IgG 抗体水平以及 IgG 和 IgA 疫苗衍生抗体水平相似。CVS 和血清中针对交叉反应性 HPV 型的疫苗衍生 IgG 抗体水平最高的是 HPV45。在 CVS 中未检测到 IgA 交叉反应性抗体反应。接种疫苗后，HPV16/18 IgG 和 IgA 抗体不仅在血清中可检测到，而且在 CVS 中也可检测到。血清和 CVS 之间 HPV16/18 IgG 抗体水平的相关性表明，疫苗诱导的 HPV 抗体从全身循环渗透和/或渗出到宫颈黏膜，以提供针对 HPV 感染的保护。
