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时间分辨荧光法（TRF）检测首段尿和血清中总 IgG 和 HPV16 特异性抗体：一项比较研究。

Time-resolved fluorescence (TRF) for total IgG and HPV16-specific antibody detection in first-void urine and serum: A comparative study.

机构信息

Center for the Evaluation of Vaccination (CEV), Vaccine & Infectious Disease Institute (VAXINFECTIO), Faculty of Medicine and Health Sciences, University of Antwerp, Wilrijk, Antwerp 2610, Belgium.

Center for the Evaluation of Vaccination (CEV), Vaccine & Infectious Disease Institute (VAXINFECTIO), Faculty of Medicine and Health Sciences, University of Antwerp, Wilrijk, Antwerp 2610, Belgium.

出版信息

J Virol Methods. 2024 Sep;329:115003. doi: 10.1016/j.jviromet.2024.115003. Epub 2024 Jul 26.

DOI:10.1016/j.jviromet.2024.115003
PMID:39069158
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11406150/
Abstract

Recent studies demonstrated that human papillomavirus (HPV) specific immunoglobulins (IgG) are present and detectable in non-invasively collected first-void urine (FVU) samples. As IgG levels in urine are low, we evaluated the potential of a highly sensitive HPV16-specific assay based on time-resolved fluorescence, DELFIA, and compared it with three immunoassays, GST-L1-MIA, M4ELISA, and M9ELISA. A total of 225 paired serum and FVU samples from two cohorts of healthy female volunteers were analyzed. Strong Spearman rank correlations between HPV16-specific IgG results measured with DELFIA, M4ELISA, GST-L1-MIA, and M9ELISA were found for both sample types (r > 0.80). Additionally, total human IgG results, determined in all samples using HTRF human IgG kit and BioPlex Pro™ Human Isotyping Assay, were compared. Moderate correlations between total human IgG concentrations in FVU samples were found for the two total IgG assays (r ≥ 0.42, p < 0.0001), while correlations for serum were non-significant. In conclusion, the HPV16-DELFIA assay is usable for detecting HPV16-specific antibodies in FVU and serum samples. As total human IgG remains an interesting parameter for the normalization of HPV-specific IgG in FVU, the accuracy of both assays needs to be validated further.

摘要

最近的研究表明，人乳头瘤病毒（HPV）特异性免疫球蛋白（IgG）存在于非侵入性采集的首次尿液（FVU）样本中，并可检测到。由于尿液中的 IgG 水平较低，我们评估了一种基于时间分辨荧光的高度敏感的 HPV16 特异性检测方法（DELFI A）的潜力，并将其与三种免疫分析方法（GST-L1-MIA、M4ELISA 和 M9ELISA）进行了比较。共分析了来自两个健康女性志愿者队列的 225 对血清和 FVU 样本。对于两种样本类型，DELFI A、M4ELISA、GST-L1-MIA 和 M9ELISA 测量的 HPV16 特异性 IgG 结果之间均存在很强的斯皮尔曼等级相关（r>0.80）。此外，使用 HTRF 人类 IgG 试剂盒和 BioPlex ProTM 人类分型分析试剂盒在所有样本中测定了总人 IgG 结果。在 FVU 样本中，两种总 IgG 测定方法的总人 IgG 浓度之间存在中度相关性（r≥0.42，p<0.0001），而血清的相关性不显著。总之，HPV16-DELFI A 检测法可用于检测 FVU 和血清样本中的 HPV16 特异性抗体。由于总人 IgG 仍然是 FVU 中 HPV 特异性 IgG 归一化的一个有趣参数，因此需要进一步验证这两种检测方法的准确性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68b3/11406150/f427a528f72f/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68b3/11406150/7d7c1367f884/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68b3/11406150/f172bb7a4e76/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68b3/11406150/a28ea982917b/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68b3/11406150/91b08b7307ab/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68b3/11406150/c5fc29097182/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68b3/11406150/f427a528f72f/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68b3/11406150/7d7c1367f884/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68b3/11406150/f172bb7a4e76/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68b3/11406150/a28ea982917b/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68b3/11406150/91b08b7307ab/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68b3/11406150/c5fc29097182/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68b3/11406150/f427a528f72f/gr6.jpg

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本文引用的文献

1
HPV-specific antibodies in female genital tract secretions captured via first-void urine retain their neutralizing capacity.通过首次尿潴留收集的女性生殖道分泌物中的 HPV 特异性抗体保留其中和能力。
Hum Vaccin Immunother. 2024 Dec 31;20(1):2330168. doi: 10.1080/21645515.2024.2330168. Epub 2024 Apr 3.
2
Follow-up of humoral immune response after HPV vaccination using first-void urine: A longitudinal cohort study.利用首段尿液检测 HPV 疫苗接种后的体液免疫反应随访：一项纵向队列研究。
J Med Virol. 2023 Oct;95(10):e29133. doi: 10.1002/jmv.29133.
3
Analytical and clinical performance of extended HPV genotyping with BD Onclarity HPV Assay in home-collected first-void urine: A diagnostic test accuracy study.
BD Onclarity HPV 检测试剂盒在家庭自采首段尿样中对 HPV 基因分型的分析和临床性能：一项诊断准确性研究。
J Clin Virol. 2022 Oct;155:105271. doi: 10.1016/j.jcv.2022.105271. Epub 2022 Aug 24.
4
Diagnostic Test Accuracy of First-Void Urine Human Papillomaviruses for Presence Cervical HPV in Women: Systematic Review and Meta-Analysis.首段尿样 HPV 检测用于女性宫颈 HPV 感染的诊断准确性：系统评价和荟萃分析。
Int J Environ Res Public Health. 2021 Dec 17;18(24):13314. doi: 10.3390/ijerph182413314.
5
The effects of the national HPV vaccination programme in England, UK, on cervical cancer and grade 3 cervical intraepithelial neoplasia incidence: a register-based observational study.英国英格兰国家 HPV 疫苗接种计划对宫颈癌和 3 级宫颈上皮内瘤变发病率的影响：基于登记的观察性研究。
Lancet. 2021 Dec 4;398(10316):2084-2092. doi: 10.1016/S0140-6736(21)02178-4. Epub 2021 Nov 3.
6
Multiplex immunoassay to measure antibody response to nine HPV vaccine types.九价人乳头瘤病毒疫苗型抗体反应的多重免疫分析检测
J Immunol Methods. 2021 Nov;498:113136. doi: 10.1016/j.jim.2021.113136. Epub 2021 Aug 28.
7
Cervical cancer screening using HPV tests on self-samples: attitudes and preferences of women participating in the VALHUDES study.使用人乳头瘤病毒（HPV）自我采样检测进行宫颈癌筛查：参与VALHUDES研究的女性的态度和偏好
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9
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