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通过透射光学杠杆法对细胞运动的光电二极管测量进行校准。

Calibration of photodiode measurements of cell motion by a transmission optical lever method.

作者信息

Clark B A, Hallworth R, Evans B N

机构信息

Department of Neurobiology and Physiology, Northwestern University, Evanston, IL 60208.

出版信息

Pflugers Arch. 1990 Jan;415(4):490-3. doi: 10.1007/BF00373629.

DOI:10.1007/BF00373629
PMID:2315008
Abstract

A method of calibrating photodiode measurements of cell motion is described. A transparent glass plane is inserted perpendicular to the light path, in front of the photodiode. When the glass plane is rotated, changing the angle between the plane and the light path, the image is displaced. The rotatable glass plane acts as an optical lever to simulate cell motion. The optical lever is easy to construct and offers excellent linearity and accuracy.

摘要

描述了一种校准光电二极管对细胞运动测量的方法。在光电二极管前方,插入一块垂直于光路的透明玻璃平面。当玻璃平面旋转时,改变平面与光路之间的角度,图像就会发生位移。可旋转的玻璃平面充当光学杠杆来模拟细胞运动。该光学杠杆易于构建,并且具有出色的线性度和精度。

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本文引用的文献

1
Microscopic observation of cochlear hair cell motility.
Scan Electron Microsc. 1984(Pt 3):1401-6.
2
The mechanical properties of ciliary bundles of turtle cochlear hair cells.龟耳蜗毛细胞纤毛束的力学特性。
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3
An ultrasensitive transducer for measurement of isometric contractile force from single heart cells.一种用于测量单个心肌细胞等长收缩力的超灵敏换能器。
Pflugers Arch. 1986 Jul;407(1):109-15. doi: 10.1007/BF00580730.
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Stereocilia displacement induced somatic motility of cochlear outer hair cells.静纤毛位移诱发耳蜗外毛细胞的体细胞运动。
Proc Natl Acad Sci U S A. 1993 Sep 15;90(18):8347-51. doi: 10.1073/pnas.90.18.8347.
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A fast motile response in guinea-pig outer hair cells: the cellular basis of the cochlear amplifier.豚鼠外毛细胞中的快速运动反应:耳蜗放大器的细胞基础。
J Physiol. 1987 Jul;388:323-47. doi: 10.1113/jphysiol.1987.sp016617.
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Photoelectric recording of mechanical responses of cardiac myocytes.心肌细胞机械反应的光电记录。
Pflugers Arch. 1987 Apr;408(4):390-4. doi: 10.1007/BF00581134.
6
Compliance of the hair bundle associated with gating of mechanoelectrical transduction channels in the bullfrog's saccular hair cell.牛蛙球囊毛细胞中与机械电转导通道门控相关的毛束顺应性。
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