Meyer R, Wiemer J, Dembski J, Haas H G
Pflugers Arch. 1987 Apr;408(4):390-4. doi: 10.1007/BF00581134.
A method to monitor contraction of isolated myocytes by transmicroscopic photometry is illustrated. Two photodiodes are mounted inside an inverse microscope used for visual control of a cell. Illumination of one diode varies in proportion to changes in cell length. The contraction signal is amplified in a comparator circuit. Spatial resolution of the device is in the order of 1 micron which corresponds to about 5% of cell shortening in the fully activated state of contraction. The method was tested on isolated myocytes from guinea-pig ventricle. Optical records of contraction in response to action potentials or during voltage clamp compare well with the contractile behavior of multicellular preparations.
阐述了一种通过透显微镜光度法监测分离心肌细胞收缩的方法。两个光电二极管安装在一台用于细胞视觉控制的倒置显微镜内。一个二极管的光照强度随细胞长度的变化成比例变化。收缩信号在比较器电路中放大。该装置的空间分辨率约为1微米，这相当于在完全激活的收缩状态下细胞缩短约5%。该方法在豚鼠心室分离的心肌细胞上进行了测试。对动作电位或电压钳制期间收缩的光学记录与多细胞制剂的收缩行为比较良好。
