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Extraction of cellular DNA from crude cell lysate with glass.

作者信息

Thompson J D, Cuddy K K, Haines D S, Gillepsie D

机构信息

Hahnemann University, Department of Neoplastic Diseases, Philadelphia, PA 19102.

出版信息

Nucleic Acids Res. 1990 Feb 25;18(4):1074. doi: 10.1093/nar/18.4.1074.

DOI:10.1093/nar/18.4.1074
PMID:2315031
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC330391/
Abstract
摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5e6/330391/56172ae72b30/nar00188-0363-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5e6/330391/56172ae72b30/nar00188-0363-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5e6/330391/56172ae72b30/nar00188-0363-a.jpg

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本文引用的文献

1
A procedure for the large-scale isolation of highly purified plasmid DNA using alkaline extraction and binding to glass powder.一种使用碱提取法并结合玻璃粉进行大规模分离高纯度质粒DNA的方法。
Anal Biochem. 1982 Apr;121(2):382-7. doi: 10.1016/0003-2697(82)90497-3.
2
Definition of a second dimeric subfamily of human alpha satellite DNA.人类α卫星DNA第二个二聚体亚家族的定义。
Nucleic Acids Res. 1989 Apr 11;17(7):2769-82. doi: 10.1093/nar/17.7.2769.
3
Preparative and analytical purification of DNA from agarose.从琼脂糖中制备和分析纯化DNA。
核酸提取效率存在高度的样本间差异，因此需要使用内标对照来基于qPCR准确测量血浆游离DNA水平。
Lab Med. 2017 Nov 8;48(4):332-338. doi: 10.1093/labmed/lmx043.
4
DNA extraction procedures meaningfully influence qPCR-based mtDNA copy number determination.DNA提取程序对基于qPCR的线粒体DNA拷贝数测定有显著影响。
Mitochondrion. 2009 Jul;9(4):261-5. doi: 10.1016/j.mito.2009.03.003. Epub 2009 Mar 24.
5
Restriction endonuclease analysis of clinical Pseudomonas aeruginosa strains: useful epidemiologic data from a simple and rapid method.
J Clin Microbiol. 1993 Jun;31(6):1426-9. doi: 10.1128/jcm.31.6.1426-1429.1993.
6
Revised interpretation of oxacillin MICs for Staphylococcus epidermidis based on mecA detection.基于mecA检测对表皮葡萄球菌苯唑西林MICs的修订解读
Antimicrob Agents Chemother. 1995 Apr;39(4):982-4. doi: 10.1128/AAC.39.4.982.
Proc Natl Acad Sci U S A. 1979 Feb;76(2):615-9. doi: 10.1073/pnas.76.2.615.