Thompson J D, Sylvester J E, Gonzalez I L, Costanzi C C, Gillespie D
Department of Neoplastic Diseases, Hahnemann University, Philadelphia, PA 19102.
Nucleic Acids Res. 1989 Apr 11;17(7):2769-82. doi: 10.1093/nar/17.7.2769.
We describe a new human subfamily of alpha satellite DNA. The restriction endonuclease XbaI cleaves this subfamily into a collection of fragments which are heterogeneous with respect to size. We compared the sequences of 6 clones from four different XbaI size classes. Clones from a single size class were not necessarily more related than clones from different classes. Clones from different size classes were found to produce almost identical hybridization patterns with XbaI-digested human genomic DNA. All clones were found to share a common dimeric repeat organization, with dimers exhibiting about 84% sequence identities, indicating that the clones evolved from a common progenitor alphoid dimer. We show that this subfamily, and the EcoRI dimer subfamily originally described by Wu and Manuelidis, evolved from different progenitor alphoid dimers, and therefore represent distinct human alphoid subfamilies.
我们描述了一个新的α卫星DNA人类亚家族。限制性内切酶XbaI将该亚家族切割成一系列大小各异的片段。我们比较了来自四个不同XbaI大小类别的6个克隆的序列。来自单个大小类别的克隆不一定比来自不同类别的克隆具有更高的相关性。发现来自不同大小类别的克隆与XbaI消化的人类基因组DNA产生几乎相同的杂交模式。所有克隆都具有共同的二聚体重复结构，二聚体表现出约84%的序列同一性，表明这些克隆是从一个共同的祖先α卫星二聚体进化而来的。我们表明，这个亚家族以及最初由Wu和Manuelidis描述的EcoRI二聚体亚家族，是从不同的祖先α卫星二聚体进化而来的，因此代表了不同的人类α卫星亚家族。
