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ColE1质粒的DNA链起始序列在单链DNA噬菌体中的表达。

Expression of a DNA strand initiation sequence of ColE1 plasmid in a single-stranded DNA phage.

作者信息

Nomura N, Ray D S

出版信息

Proc Natl Acad Sci U S A. 1980 Nov;77(11):6566-70. doi: 10.1073/pnas.77.11.6566.

DOI:10.1073/pnas.77.11.6566
PMID:7005899
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC350326/
Abstract

In order to investigate initiation of H-strand (lagging strand) replication of the plasmid ColE1, the origin region fragment (Hae II-E) of ColE1 was inserted into the intergenic region of filamentous DNA phage M13 and cloned. A site capable of promoting DNA strand initiation on a single-stranded DNA template has been detected on the L-strand (leading strand) of the cloned fragment. The site, named rri-1 rifampicin-resistant initiation), directs conversion of chimeric phage single-stranded DNA to parental replicative form in the presence of rifampicin, which blocks the function of the complementary strand origin of M13. The function of rri-1 is dependent on both the dnaG and dnaB gene products. It is postulated that rri-1 might be an initiation site for synthesis of the lagging DNA strand during unidirectional replication of ColE1 DNA.

摘要

为了研究质粒ColE1的H链（后随链）复制的起始过程，将ColE1的起始区域片段（Hae II-E）插入丝状DNA噬菌体M13的基因间隔区并进行克隆。在克隆片段的L链（前导链）上检测到一个能够在单链DNA模板上促进DNA链起始的位点。该位点名为rri-1（利福平抗性起始），在利福平存在的情况下，可指导嵌合噬菌体单链DNA转化为亲本复制型，利福平会阻断M13互补链起始位点的功能。rri-1的功能依赖于dnaG和dnaB基因产物。据推测，rri-1可能是ColE1 DNA单向复制过程中滞后DNA链合成的起始位点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b4e1/350326/45ba3e8e96a7/pnas00498-0320-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b4e1/350326/8c9e4548a1de/pnas00498-0320-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b4e1/350326/9011f474955c/pnas00498-0320-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b4e1/350326/8e11347664dc/pnas00498-0320-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b4e1/350326/45ba3e8e96a7/pnas00498-0320-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b4e1/350326/8c9e4548a1de/pnas00498-0320-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b4e1/350326/9011f474955c/pnas00498-0320-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b4e1/350326/8e11347664dc/pnas00498-0320-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b4e1/350326/45ba3e8e96a7/pnas00498-0320-d.jpg

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本文引用的文献

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Plasmid ColE1 DNA replication in Escherichia coli strains temperature-sensitive for DNA replication.在对DNA复制温度敏感的大肠杆菌菌株中质粒ColE1 DNA的复制。
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An Escherichia coli replication protein that recognizes a unique sequence within a hairpin region in phi X174 DNA.一种能识别φX174 DNA发夹区域内独特序列的大肠杆菌复制蛋白。
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Identification of a primosome assembly site in the region of the ori 2 replication origin of the Escherichia coli mini-F plasmid.在大肠杆菌微型F质粒ori 2复制起点区域中鉴定引发体装配位点。
Proc Natl Acad Sci U S A. 1983 Dec;80(23):7132-6. doi: 10.1073/pnas.80.23.7132.
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Viable deletions of the M13 complementary strand origin.M13互补链起始位点的可行缺失。
Proc Natl Acad Sci U S A. 1981 Nov;78(11):6784-8. doi: 10.1073/pnas.78.11.6784.
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Escherichia coli factor Y sites of plasmid pBR322 can function as origins of DNA replication.质粒pBR322的大肠杆菌Y因子位点可作为DNA复制的起始点。
Proc Natl Acad Sci U S A. 1981 Oct;78(10):6111-5. doi: 10.1073/pnas.78.10.6111.
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Rifampicin-resistant initiation of DNA synthesis on the isolated strands of ColE plasmid DNA.在ColE质粒DNA的单链上，利福平抗性的DNA合成起始。
Nucleic Acids Res. 1981 Oct 24;9(20):5215-31. doi: 10.1093/nar/9.20.5215.
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The nucleotide sequence of the replication origin of plasmid NTP1.质粒NTP1复制起点的核苷酸序列。
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新型大肠杆菌噬菌体M13克隆载体的构建与特性分析
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A possible role for RNA polymerase in the initiation of M13 DNA synthesis.RNA聚合酶在M13 DNA合成起始过程中的可能作用。
Proc Natl Acad Sci U S A. 1971 Nov;68(11):2826-9. doi: 10.1073/pnas.68.11.2826.
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Replication of bacteriophage M13. VI. Attachment of M13 DNA to a fast-sedimenting host cell component.噬菌体M13的复制。VI. M13 DNA与快速沉降的宿主细胞成分的附着
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Biochim Biophys Acta. 1969 Apr 22;179(2):398-407.
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Unidirectional replication of plasmid ColE1 DNA.质粒ColE1 DNA的单向复制。
Nature. 1974 Sep 27;251(5473):337-40. doi: 10.1038/251337a0.
10
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