ColE1质粒的DNA链起始序列在单链DNA噬菌体中的表达。

Proc Natl Acad Sci U S A. 1980 Nov;77(11):6566-70. doi: 10.1073/pnas.77.11.6566.

In order to investigate initiation of H-strand (lagging strand) replication of the plasmid ColE1, the origin region fragment (Hae II-E) of ColE1 was inserted into the intergenic region of filamentous DNA phage M13 and cloned. A site capable of promoting DNA strand initiation on a single-stranded DNA template has been detected on the L-strand (leading strand) of the cloned fragment. The site, named rri-1 rifampicin-resistant initiation), directs conversion of chimeric phage single-stranded DNA to parental replicative form in the presence of rifampicin, which blocks the function of the complementary strand origin of M13. The function of rri-1 is dependent on both the dnaG and dnaB gene products. It is postulated that rri-1 might be an initiation site for synthesis of the lagging DNA strand during unidirectional replication of ColE1 DNA.

为了研究质粒ColE1的H链（后随链）复制的起始过程，将ColE1的起始区域片段（Hae II-E）插入丝状DNA噬菌体M13的基因间隔区并进行克隆。在克隆片段的L链（前导链）上检测到一个能够在单链DNA模板上促进DNA链起始的位点。该位点名为rri-1（利福平抗性起始），在利福平存在的情况下，可指导嵌合噬菌体单链DNA转化为亲本复制型，利福平会阻断M13互补链起始位点的功能。rri-1的功能依赖于dnaG和dnaB基因产物。据推测，rri-1可能是ColE1 DNA单向复制过程中滞后DNA链合成的起始位点。