Faucher Sebastien P, Shuman Howard A
Faculty of Agricultural and Environmental Sciences, Department of Natural Resource Sciences, McGill University, Quebec, Canada.
Methods Mol Biol. 2013;954:567-82. doi: 10.1007/978-1-62703-161-5_35.
The study of transcriptome responses can provide insight into the regulatory pathways and genetic factors that contribute to a specific phenotype. For bacterial pathogens, it can identify putative new virulence systems and shed light on the mechanisms underlying the regulation of virulence factors. Microarrays have been previously used to study gene regulation in Legionella pneumophila. In the past few years a sharp reduction of the costs associated with microarray experiments together with the availability of relatively inexpensive custom-designed commercial microarrays has made microarray technology an accessible tool for the majority of researchers. Here we describe the methodologies to conduct microarray experiments from in vitro and in vivo samples.
转录组反应的研究能够深入了解促成特定表型的调控途径和遗传因素。对于细菌病原体而言，它可以识别潜在的新毒力系统，并揭示毒力因子调控背后的机制。微阵列先前已被用于研究嗜肺军团菌中的基因调控。在过去几年中，与微阵列实验相关的成本大幅降低，同时相对廉价的定制商业微阵列的出现，使得微阵列技术成为大多数研究人员都能使用的工具。在此，我们描述了从体外和体内样本进行微阵列实验的方法。
