Jackson Lydgia A, Dyer David W
Department of Microbiology and Immunology, The University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA.
Methods Mol Biol. 2012;903:343-57. doi: 10.1007/978-1-61779-937-2_24.
Gene expression profiling using DNA microarrays has become commonplace in current molecular biology practices, and has dramatically enhanced our understanding of the biology of Neisseria spp., and the interaction of these organisms with the host. With the choice of microarray platforms offered for gene expression profiling and commercially available arrays, investigators must ask several central questions to make decisions based on their research focus. Are arrays on hand for their organism and if not then would it be cost-effective to design custom arrays. Other important considerations; what types of specialized equipment for array hybridization and signal detection are required and is the specificity and sensitivity of the array adequate for your application. Here, we describe the use of a custom 12K CombiMatrix ElectraSense™ oligonucleotide microarray format for assessing global gene expression profiles in Neisseria spp.
使用DNA微阵列进行基因表达谱分析在当前分子生物学实践中已变得很常见，并且极大地增进了我们对奈瑟菌属生物学以及这些生物体与宿主相互作用的理解。随着用于基因表达谱分析的微阵列平台以及市售阵列的选择，研究人员必须提出几个核心问题，以便根据他们的研究重点做出决策。手头是否有针对其研究生物体的阵列，如果没有，设计定制阵列是否具有成本效益。其他重要考虑因素包括：进行阵列杂交和信号检测需要哪些类型的专用设备，以及阵列的特异性和灵敏度是否足以满足您的应用需求。在这里，我们描述了使用定制的12K CombiMatrix ElectraSense™寡核苷酸微阵列格式来评估奈瑟菌属中的全局基因表达谱。
