Löser C, Fölsch U R, Paprotny C, Creutzfeldt W
Department of Medicine, Georg August-University of Göttingen, F.R.G.
Pancreas. 1990 Mar;5(2):119-27. doi: 10.1097/00006676-199003000-00001.
We investigated the total, free, and acetylated polyamine concentrations in pancreatic tissue, serum, and urine of 20 patients with pancreatic cancer, 30 healthy volunteers, and 40 patients with nonmalignant, gastrointestinal diseases by reversed-phase liquid chromatography. Tissue concentrations in carcinoma compared to histologically unaffected pancreas were significantly higher for putrescine, elevated for cadaverine, and nearly identical for spermidine and spermine, while N1-acetylspermidine was detectable in cancer tissue only. With the exception of free spermine in urine and total spermine in serum, all other polyamines were significantly elevated in the urine and serum of cancer patients compared to healthy controls. These data support the concept that polyamines play an important role in rapidly growing tissues. However, nonmalignant gastrointestinal diseases partly showed similar elevations. Because of this low specificity, polyamines are of little value only as diagnostic markers of pancreatic carcinoma. Since polyamine concentrations normalized in patients after curative operation while they were further elevated in patients with tumor relapse or metastases, polyamines might play a clinical role in predicting therapeutic success or indicating relapse of the tumor. A significant linear correlation of polyamine concentrations and the size of the tumor was found while a significant correlation to CEA, CA 19-9, and CA 125 or the presence of organ metastases did not exist.
我们采用反相液相色谱法，对20例胰腺癌患者、30名健康志愿者以及40例患有非恶性胃肠道疾病的患者的胰腺组织、血清和尿液中的总多胺、游离多胺及乙酰化多胺浓度进行了研究。与组织学上未受影响的胰腺相比，癌组织中腐胺的浓度显著升高，尸胺浓度有所升高，亚精胺和精胺浓度几乎相同，而N1-乙酰亚精胺仅在癌组织中可检测到。与健康对照组相比，除了尿液中的游离精胺和血清中的总精胺外，癌症患者尿液和血清中的所有其他多胺均显著升高。这些数据支持多胺在快速生长组织中起重要作用的观点。然而，非恶性胃肠道疾病部分也表现出类似的升高。由于这种低特异性，多胺仅作为胰腺癌的诊断标志物价值不大。由于根治性手术后患者的多胺浓度恢复正常，而肿瘤复发或转移患者的多胺浓度进一步升高，因此多胺可能在预测治疗效果或提示肿瘤复发方面发挥临床作用。我们发现多胺浓度与肿瘤大小呈显著线性相关，而与癌胚抗原（CEA）、糖类抗原19-9（CA 19-9）和糖类抗原125（CA 125）或器官转移的存在不存在显著相关性。
