Department of Nutrition, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, Oslo, Norway.
Prostaglandins Leukot Essent Fatty Acids. 2013 Feb;88(2):155-62. doi: 10.1016/j.plefa.2012.10.001. Epub 2012 Nov 13.
Fatty acids regulate angiogenesis although no such information is available in first trimester placental trophoblast cells despite the fact that angiogenesis is a critical step involving these cells in early placentation. We investigated effects of different fatty acids on angiogenesis, their uptake and metabolism and expression of lipid metabolic genes in first trimester placental trophoblast cells using HTR-8/SVneo cell line. Fatty acid uptake by these cells exhibited a saturable kinetics. Uptake of AA was consistently greater compared with that of EPA and DHA throughout the incubation period of 180 min. Use of triacsin C, an inhibitor of acyl-CoA synthetase, significantly inhibited fatty acid uptake as well as fatty acid induced cell proliferation in these cells. Angiogenic effect (as measured by tube formation) of these fatty acids was in the following order DHA> EPA> AA> OA. Angiogenic effect of these fatty acids (AA, EPA, OA) was significantly decreased in ANGPTL4 knocked down cells, indicating ANGPTL4 may be involved at least in part in fatty acid induced angiogenesis. In addition, these fatty acids altered expression of several lipid metabolic genes such as ADRP, FABP4, FABP3, and COX-2 those are involved in angiogenesis. All these data suggest that fatty acids regulate angiogenic processes in these cells via different mechanisms.
脂肪酸调节血管生成，尽管在妊娠早期胎盘滋养层细胞中没有这样的信息，尽管血管生成是这些细胞在早期胎盘形成中涉及的关键步骤。我们使用 HTR-8/SVneo 细胞系研究了不同脂肪酸对血管生成、摄取和代谢以及脂质代谢基因表达的影响。这些细胞的脂肪酸摄取表现出饱和动力学。在 180 分钟的孵育期间，AA 的摄取始终大于 EPA 和 DHA。使用酰基辅酶 A 合成酶抑制剂三碘乙酸 C，可显著抑制这些细胞中的脂肪酸摄取和脂肪酸诱导的细胞增殖。这些脂肪酸的血管生成效应（如管形成测量）的顺序为 DHA> EPA> AA> OA。在 ANGPTL4 敲低的细胞中，这些脂肪酸（AA、EPA、OA）的血管生成效应显著降低，表明 ANGPTL4 至少部分参与了脂肪酸诱导的血管生成。此外，这些脂肪酸改变了参与血管生成的几个脂质代谢基因的表达，如 ADRP、FABP4、FABP3 和 COX-2。所有这些数据表明，脂肪酸通过不同的机制调节这些细胞中的血管生成过程。
