College of Chemistry, Liaoning University, Shenyang 110036, PR China.
Ultrason Sonochem. 2013 Mar;20(2):685-95. doi: 10.1016/j.ultsonch.2012.10.008. Epub 2012 Oct 27.
The interaction of Cresol Red derivatives (Cresol Red (o-Cresolsulfonphthalein), Cresol Red-DA (3,3'-Bis [N,N-di (carboxymethyl) aminomethyl]-o-cresolsulfonphthalein) and Cresol Red-DA-Fe(III) (3,3'-Bis [N,N-di (carboxymethyl) aminomethyl]-o-cresolsulfonphthalein-Ferrous(III)) with bovine serum albumin (BSA) were studied by the combination of ultraviolet spectroscopy, circular dichroism (CD) spectroscopy, fluorescence spectroscopy and synchronous fluorescence spectroscopy. On that basis, the sonodynamic and sonocatalytic damages of Cresol Red derivatives to BSA under ultrasonic irradiation were also investigated by means of corresponding spectrum technology. Meanwhile, some influenced factors such as ultrasonic irradiation time, Cresol Red derivatives concentration and ionic strength on the damage degree of BSA molecules were also reviewed. In addition, the binding site and damage site of BSA molecules were estimated by synchronous fluorescence spectra. Finally, the results of oxidation-extraction photometry (OEP) using several reactive oxygen species (ROS) scavengers indicated that the damage of BSA molecules is mainly due to the generation of ROS. Perhaps, this paper may offer some important subjects for broadening the application of Cresol Red derivatives in sonodynamic therapy (SDT) and sonocatalytic therapy (SCT) technologies for tumor treatment.
邻甲酚红衍生物（邻甲酚红（邻甲酚磺酰酞）、邻甲酚红-DA（3,3'-双[N,N-二（羧甲基）氨甲基]-邻甲酚磺酰酞）和邻甲酚红-DA-Fe(III)（3,3'-双[N,N-二（羧甲基）氨甲基]-邻甲酚磺酰酞-亚铁（III））与牛血清白蛋白（BSA）的相互作用通过紫外光谱、圆二色性（CD）光谱、荧光光谱和同步荧光光谱进行研究。在此基础上，还通过相应的光谱技术研究了超声辐射下邻甲酚红衍生物对 BSA 的声动力学和声催化损伤。同时，考察了超声辐照时间、邻甲酚红衍生物浓度和离子强度等因素对 BSA 分子损伤程度的影响。此外，通过同步荧光光谱估算了 BSA 分子的结合位点和损伤位点。最后，使用几种活性氧（ROS）清除剂的氧化萃取光度法（OEP）结果表明，BSA 分子的损伤主要归因于 ROS 的产生。也许，本文可以为拓宽邻甲酚红衍生物在声动力学疗法（SDT）和声催化疗法（SCT）技术治疗肿瘤中的应用提供一些重要课题。
