Department of Pharmacy, Liaoning University, Shenyang 110036, PR China.
J Photochem Photobiol B. 2010 Jan 21;98(1):61-8. doi: 10.1016/j.jphotobiol.2009.11.005. Epub 2009 Nov 22.
By research, it was found that the Amsacrine (AMSA) can interact with bovine serum albumin (BSA). In this work, the AMSA was adopted as a sonosensitizer and the Metronidazole (MET) was used as a sensitizer to further damage BSA molecules under ultrasonic irradiation. It could be concluded that the damage degree of BSA molecules in the presence of AMSA and MET was more serious than in the presence of pure AMSA. That is, MET could aggravate the damage to BSA molecules under ultrasonic irradiation combined with AMSA. Meanwhile, the damage degree of BSA molecules was also influenced by some factors, such as ultrasonic irradiation time, MET concentration and solution acidity. In addition, the damage site of BSA molecules was estimated by synchronous fluorescence spectra. It was found that the tyrosine (Tyr) and tryptophan (Typ) residues were damaged almost averagely. Perhaps, these research results are of great significance for driving sonodynamic method to treat tumor in clinic application.
通过研究发现吖啶酮（AMSA）可以与牛血清白蛋白（BSA）相互作用。在这项工作中，采用吖啶酮（AMSA）作为声敏剂，甲硝唑（MET）作为敏化剂，在超声辐射下进一步破坏 BSA 分子。可以得出结论，在存在 AMSA 和 MET 的情况下，BSA 分子的损伤程度比单独存在 AMSA 时更严重。也就是说，MET 可以加剧超声辐射与 AMSA 联合作用下对 BSA 分子的损伤。同时，BSA 分子的损伤程度还受到超声辐照时间、MET 浓度和溶液酸度等因素的影响。此外，通过同步荧光光谱估计了 BSA 分子的损伤部位。结果发现，色氨酸（Tyr）和色氨酸（Typ）残基几乎平均受到损伤。也许，这些研究结果对于推动声动力学方法在临床应用中治疗肿瘤具有重要意义。
