College of Chemistry, Liaoning University, Shenyang 110036, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2012 Feb 15;87:1-10. doi: 10.1016/j.saa.2011.11.006. Epub 2011 Nov 20.
In this paper, the riboflavin (RF) was used to study the interaction and sonodynamic damage to bovine serum albumin (BSA) by fluorescence and UV-vis spectroscopy. The results showed that the RF could efficiently bind to BSA in aqueous solution. Under ultrasonic irradiation, the RF could obviously damage the BSA. In addition, synchronous fluorescence spectroscopy revealed that the RF showed more accessible to tryptophan (Trp) residues than to tyrosine (Tyr) residues. Also, it damaged Trp residues more seriously than Tyr residues under ultrasonic irradiation. At last, the generation of reactive oxygen species (ROS) in sonodynamic process was estimated by the method of Oxidation-Extraction Spectrometry (OES). And then, several radical scavengers were used to determine the kind of ROS. It was found that at least the singlet oxygen ((1)O(2)) and hydroxyl radicals (*OH) were generated.
本文使用核黄素（RF）通过荧光和紫外可见光谱研究其与牛血清白蛋白（BSA）的相互作用和超声动力学损伤。结果表明，RF 可以在水溶液中有效地与 BSA 结合。在超声辐射下，RF 可以明显破坏 BSA。此外，同步荧光光谱表明，与 Tyr 残基相比，RF 更易接近色氨酸（Trp）残基。而且，在超声辐射下，RF 对 Trp 残基的损伤比 Tyr 残基更严重。最后，通过氧化萃取光谱法（OES）来估算超声动力学过程中活性氧（ROS）的产生。然后，使用几种自由基清除剂来确定 ROS 的种类。结果发现，至少生成了单线态氧（(1)O(2)）和羟基自由基（*OH）。
