Astbury Centre for Structural Molecular Biology, University of Leeds, Woodhouse Lane, Leeds, LS2 9JT, UK.
School of Electronic and Electrical Engineering, University of Leeds, Woodhouse Lane, Leeds, LS2 9JT, UK.
Nanotechnology. 2012 Dec 14;23(49):495304. doi: 10.1088/0957-4484/23/49/495304. Epub 2012 Nov 16.
Numerous nanoscale devices and materials have been fabricated in recent years using a variety of biological scaffolds. However, the interfacing of these devices and materials into existing circuits and ordered arrays has proved problematic. Here, we describe a simple solution to this problem using self-assembly of the peptide coiled-coil heterodimer ACID:BASE to immobilize M13 bacteriophage particles to specific locations on a patterned gold surface. Surface plasmon resonance demonstrated that free ACID peptides will assemble onto a surface derivatized with BASE. We then displayed the ACID peptide on the pIX coat protein of M13 and showed that these phage particles permit formation of the coiled-coil resulting in specific surface attachment. The ACID:immobilized BASE affinities appear to be similar for free peptide and phage-displayed ACID. Finally, we fabricated two gold electrodes, separated by a 200 nm gap, coated one of them with BASE and showed that this allows localization of the M13:ACID onto the functionalized electrode.
近年来，人们使用各种生物支架制造了许多纳米级器件和材料。然而，将这些器件和材料与现有的电路和有序阵列进行接口连接一直是个问题。在这里，我们描述了一种使用自组装酸-碱二聚体 ACID:BASE 肽螺旋来将 M13 噬菌体颗粒固定在图案化金表面特定位置的简单解决方案。表面等离子体共振表明，游离的 ACID 肽会组装到 BASE 衍生的表面上。然后，我们将 ACID 肽展示在 M13 的 pIX 外壳蛋白上，并表明这些噬菌体颗粒允许形成螺旋，从而导致特定的表面附着。游离肽和噬菌体展示的 ACID 的 ACID:固定 BASE 亲和力似乎相似。最后，我们制造了两个金电极，它们之间有 200nm 的间隙，其中一个电极涂有 BASE，并表明这允许 M13:ACID 定位于功能化电极上。
