Department of Biological Chemistry and Molecular Modeling, IQAC (CSIC), Barcelona, Spain.
PLoS One. 2012;7(11):e47751. doi: 10.1371/journal.pone.0047751. Epub 2012 Nov 14.
Infochemical production, release and detection of (Z,E)-9,11-tetradecadienyl acetate, the major component of the pheromone of the moth Spodoptera littoralis, is achieved in a novel microfluidic system designed to mimic the final step of the pheromone biosynthesis by immobilized recombinant alcohol acetyl transferase. The microfluidic system is part of an "artificial gland", i.e., a chemoemitter that comprises a microreactor connected to a microevaporator and is able to produce and release a pre-defined amount of the major component of the pheromone from the corresponding (Z,E)-9,11-tetradecadienol. Performance of the entire chemoemitter has been assessed in electrophysiological and behavioral experiments. Electroantennographic depolarizations of the pheromone produced by the chemoemitter were ca. 40% relative to that evoked by the synthetic pheromone. In a wind tunnel, the pheromone released from the evaporator elicited on males a similar attraction behavior as 3 virgin females in most of the parameters considered.
（Z，E）-9，11-十四碳二烯基乙酸酯是鳞翅目夜蛾 Spodoptera littoralis 信息素的主要成分，其在新型微流控系统中的产生、释放和检测是通过固定化重组醇乙酰转移酶来模拟信息素生物合成的最后一步实现的。该微流控系统是“人工腺体”的一部分，即化学发射器，由与微蒸发器相连的微反应器组成，能够从相应的（Z，E）-9，11-十四碳二烯醇产生并释放预定量的信息素主要成分。整个化学发射器的性能已通过电生理和行为实验进行了评估。化学发射器产生的信息素引起的触角电位去极化约为合成信息素引起的 40%。在风洞中，从蒸发器释放的信息素在大多数考虑的参数上引起雄性类似的吸引行为，与 3 只处女雌性相当。
