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Mycoplasma mycoides subsp. capri 中的 III 型限制修饰系统。

A Type III restriction-modification system in Mycoplasma mycoides subsp. capri.

机构信息

The J. Craig Venter Institute, 9704 Medical Center Drive, Rockville, MD 20850, USA.

出版信息

Open Biol. 2012 Oct;2(10):120115. doi: 10.1098/rsob.120115.

DOI:10.1098/rsob.120115
PMID:23155485
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3498834/
Abstract

The sequenced genome of Mycoplasma mycoides subsp. capri revealed the presence of a Type III restriction-modification system (MmyCI). The methyltransferase (modification) subunit of MmyCI (M.MmyCI) was shown to recognize the sequence 5'-TGAG-3' and methylate the adenine. The coding region of the methyltransferase gene contains 12 consecutive AG dinucleotide repeats that result in a translational termination at a TAA codon immediately beyond the repeat region. This strain does not have MmyCI activity. A clone was found with 10 AG repeats such that the gene is in frame, and this strain has MmyCI activity, suggesting that the expression of the MmyCI methyltransferase may be phase variable.

摘要

绵羊肺炎支原体亚种 capri 的测序基因组揭示了一种 III 型限制修饰系统（MmyCI）的存在。MmyCI 的甲基转移酶（修饰）亚基（M.MmyCI）被证明能识别序列 5'-TGAG-3'并使腺嘌呤甲基化。甲基转移酶基因的编码区含有 12 个连续的 AG 二核苷酸重复序列，导致在重复区域之后的 TAA 密码子处立即发生翻译终止。该菌株没有 MmyCI 活性。发现了一个具有 10 个 AG 重复的克隆，使得基因成框，并且该菌株具有 MmyCI 活性，表明 MmyCI 甲基转移酶的表达可能是相变异的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb08/3498834/8a98a2c14541/rsob-2-120115-g4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb08/3498834/b6f641f396c7/rsob-2-120115-g1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb08/3498834/e31c86d91764/rsob-2-120115-g2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb08/3498834/695d6471405a/rsob-2-120115-g3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb08/3498834/8a98a2c14541/rsob-2-120115-g4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb08/3498834/b6f641f396c7/rsob-2-120115-g1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb08/3498834/e31c86d91764/rsob-2-120115-g2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb08/3498834/695d6471405a/rsob-2-120115-g3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb08/3498834/8a98a2c14541/rsob-2-120115-g4.jpg

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