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西班牙美国扁柏精油的抗真菌和抗菌活性

Antifungal and antibacterial activity of the essential oil of Chamaecyparis lawsoniana from Spain.

作者信息

Palá-Paúl Jesús, Usano-Alemany Jaime, Granda Elena, Soria Ana-Cristina

机构信息

Dpto. Biologia Vegetal I (Botcinica), Facultad de Biologia, Universidad Complutense de Madrid, 28040-Madrid, Spain.

出版信息

Nat Prod Commun. 2012 Oct;7(10):1383-6.

PMID:23157017
Abstract

The essential oils extracted from the young stems and leaves of Chamaecyparis lawsoniana (A.Murray) Parl. have been analysed by Gas Chromatography and Gas Chromatography coupled to Mass Spectrometry. A total of 66 compounds were identified representing around the 99% of the total oil. The oil was richer in monoterpenes than in sesquiterpenes. The only main component was limonene with a percentage composition of 77.7%. The rest of compounds that contribute to the fragrance had percentage composition lower that the 3.0%: p-cymen-7-ol (3.0%), myrcene (2.4%), camphor (2.1%), delta-elemene (1.6%), oplopanonyl acetate (1.6%), methyl perillate (1.3%), terpinen-4-ol (1.0%) and beta-oplopenone 1.0%. The antibacterial and antifungal activity of this oil was also tested against different microorganisms. The only fungus tested, Candida albicans, was very sensitive to the treatment with an inhibition halos of 20mm. The oil was more effective with the Gram (+) than with Gram (-) bacteria. The inhibition halos were 12mm, 12-13mm and 12-13mm for Bacillus subtilis, Staphylococcus aureus and Micrococcus luteus respectively. We report new data of the antibacterial and antifungal activity of the essential oil of this species. The essential oil of C. lawsoniana could be considered as a good natural antibacterial and antifungal agent.

摘要

对美国扁柏(Chamaecyparis lawsoniana (A.Murray) Parl.)幼嫩茎叶中提取的精油进行了气相色谱和气相色谱-质谱联用分析。共鉴定出66种化合物,约占精油总量的99%。该精油中单萜类化合物比倍半萜类化合物含量更高。唯一的主要成分是柠檬烯,其百分含量为77.7%。其余对香气有贡献的化合物百分含量均低于3.0%:对伞花烃-7-醇(3.0%)、月桂烯(2.4%)、樟脑(2.1%)、δ-榄香烯(1.6%)、乙酸蛇麻酯(1.6%)、紫苏酸甲酯(1.3%)、萜品-4-醇(1.0%)和β-蛇麻烯酮(1.0%)。还测试了该精油对不同微生物的抗菌和抗真菌活性。唯一测试的真菌白色念珠菌对该处理非常敏感,抑菌圈为20毫米。该精油对革兰氏阳性菌比对革兰氏阴性菌更有效。对枯草芽孢杆菌、金黄色葡萄球菌和藤黄微球菌的抑菌圈分别为12毫米、12 - 13毫米和12 - 13毫米。我们报道了该物种精油抗菌和抗真菌活性的新数据。美国扁柏精油可被视为一种良好的天然抗菌和抗真菌剂。

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