Flye M W, Yu S
Department of Surgery, Washington University School of Medicine, St. Louis, Missouri 63110.
Transplantation. 1990 Mar;49(3):581-7. doi: 10.1097/00007890-199003000-00020.
The cytolytic responses of C3H/HeJ mice after 50% hepatectomy (PH) were assessed in a 4-hr 51Cr-release assay. Spleen cells (SC) (50 x 10(6] from normal or PH C3H/HeJ (H-2k) mice were sensitized with equal numbers of irradiated allogeneic DBA/2 (H-2d) spleen cells in a five-day mixed lymphocyte culture. Generated cytolytic activity was measured against 51Cr-labeled P815 mastocytoma (H-2d) and EL4 lymphoma (H-2b) target cells. The wet weight and cell numbers per spleen following 50% partial hepatectomy were 70% and 75% higher than the control values for the first 20 days, and then returned to normal levels by 21 days. The cytolysis by spleen cells from 2-, 14-, and 31-day PH mice were 89.3 +/- 0.7, 86.9 +/- 5.3, and 90.1 +/- 1.3%, respectively, compared with control (sham-operated) values of 56.0 +/- 1.0, 57.0 +/- 2.0, and 49.9 +/- 7.0% (P less than 0.03 at E/T 100:1). This enhanced cytolysis by PH spleen cells remained high for at least 118 days after the liver resection before returning to control levels by 268 days. Cytolytic effector cells in PH SC were generated at least 24 hr earlier than in control SC. When normal and PH cytolysis were compared following primary and secondary in vitro sensitization, the cytolytic levels of primarily-sensitized PH spleen cells were comparable to secondarily sensitized normal spleen cells. Furthermore, the primarily sensitized normal spleen cells did not show crossreactive cytolysis with EL4 target cells (H-2b), while both the primarily sensitized PH spleen cells and the secondarily sensitized normal spleen cells were significantly cross-reactive against the third party EL4 target cells. Adherent PH spleen cells appear to be responsible for this augmented cytolytic capacity since their coculture with normal nonadherent responder spleen cells increased control cytolysis by approximately 30%. These studied demonstrate that, following 50% partial hepatectomy, there is an immediate and sustained increase in the allospecific cytolytic response.
在4小时的51铬释放试验中评估了50%肝切除（PH）后C3H/HeJ小鼠的细胞溶解反应。来自正常或PH C3H/HeJ（H-2k）小鼠的脾细胞（SC）（50×10⁶）在为期五天的混合淋巴细胞培养中与等量的经照射的同种异体DBA/2（H-2d）脾细胞进行致敏。针对51铬标记的P815肥大细胞瘤（H-2d）和EL4淋巴瘤（H-2b）靶细胞测量产生的细胞溶解活性。50%部分肝切除后最初20天脾脏的湿重和细胞数量比对照值分别高70%和75%，然后在21天时恢复到正常水平。与对照（假手术）值56.0±1.0%、57.0±2.0%和49.9±7.0%相比，2天、14天和31天PH小鼠的脾细胞溶解率分别为89.3±0.7%、86.9±5.3%和90.1±1.3%（在E/T为100:1时P<0.03）。肝切除后，PH脾细胞增强的细胞溶解作用至少在118天内保持较高水平，直至268天时恢复到对照水平。PH SC中的细胞溶解效应细胞比对照SC至少提前24小时产生。当比较初次和二次体外致敏后的正常和PH细胞溶解情况时，初次致敏的PH脾细胞的细胞溶解水平与二次致敏的正常脾细胞相当。此外，初次致敏的正常脾细胞对EL4靶细胞（H-2b）未表现出交叉反应性细胞溶解，而初次致敏的PH脾细胞和二次致敏的正常脾细胞对第三方EL4靶细胞均有显著的交叉反应性。贴壁的PH脾细胞似乎是这种增强的细胞溶解能力的原因，因为它们与正常的非贴壁反应性脾细胞共培养使对照细胞溶解增加了约30%。这些研究表明，50%部分肝切除后，同种异体特异性细胞溶解反应立即且持续增加。
