Shao Yi, Jiang Yuancong, Li Hui, Zhang Feng, Hu Zhenhua, Zheng Shusen
Department of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China.
Key Laboratory of Organ Transplantation, Research Center for Diagnosis and Treatment of Hepatobiliary Diseases, Hangzhou, Zhejiang 310003, P.R. China.
Exp Ther Med. 2021 Sep;22(3):953. doi: 10.3892/etm.2021.10385. Epub 2021 Jul 5.
Dysbiosis of the gut microbiota has important roles in various diseases and pathological states of the host. However, the changes of the gut microbiota during partial hepatectomy (PH)-induced acute liver injury have so far remained elusive. The present study investigated the gut microbiome and its related pathways following PH-induced acute liver injury. A total of 50 male C57/BL6 mice were divided into a normal control (NC), sham-operation and liver resection (LR) group (50% PH). Samples were collected at 3 and 14 days post-operation to obtain specimens for the Sham3, Sham14, LR3 and LR14 groups (10 mice/group). Specimens of NC group (n=10) were obtained at the same time as those of Sham3 group. Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were determined using an automatic chemical analyzer and the gut microbiota was assessed by 16S ribosomal RNA gene sequencing of small intestinal contents. The serum levels of ALT and AST in the LR3 group were significantly increased, while those in the LR14 group were decreased again to near-normal levels. In the LR3 group, the operational taxonomic units, species richness (Chao1) and species diversity (Shannon and Simpson indices) were decreased, although without any significant difference. Furthermore, in the LR3 group, significant Cyanobacteria enrichment and Fusobacteria depletion compared with the NC and Sham3 groups was observed, while in the LR14 group, a significant depletion of the abundance of Verrucomicrobia, Chloroflexi and Deferribacteres compared to the LR3 group was obtained. The abundance of Firmicutes was increased in the LR3 group and decreased again in the LR14 group. However, the abundance of Bacteroidetes and Actinobacteria decreased in the LR3 group and increased again in the LR14 group. The alterations of the gut microbiota at the genus level were also revealed, as significant increases in , , , UCG-005 and and a sharp decrease in and were caused by acute liver injury. Furthermore, functional metagenome prediction was performed by Phylogenetic Investigation of Communities by Reconstruction of Unobserved States based on the Greengenes database, revealing alterations in signal transduction, transcription and cell motility, as well as metabolism of amino acids, lipids, glucose, cofactors and terpenoids, and xenobiotics pathways. An improved understanding of the structural and functional changes of the gut microbiota following 50% PH-induced acute liver injury and repair may provide novel strategies for the recovery of hosts undergoing hepatectomy.
肠道微生物群失调在宿主的各种疾病和病理状态中发挥着重要作用。然而，部分肝切除术（PH）诱导的急性肝损伤期间肠道微生物群的变化迄今仍不清楚。本研究调查了PH诱导的急性肝损伤后的肠道微生物组及其相关途径。总共50只雄性C57/BL6小鼠被分为正常对照组（NC）、假手术组和肝切除组（LR）（50%PH）。在术后3天和14天收集样本，以获取Sham3、Sham14、LR3和LR14组的标本（每组10只小鼠）。NC组（n = 10）的标本与Sham3组同时获取。使用自动化学分析仪测定丙氨酸氨基转移酶（ALT）和天冬氨酸氨基转移酶（AST），并通过小肠内容物的16S核糖体RNA基因测序评估肠道微生物群。LR3组的血清ALT和AST水平显著升高，而LR14组的血清ALT和AST水平再次降至接近正常水平。在LR3组中，操作分类单元、物种丰富度（Chao1）和物种多样性（香农和辛普森指数）有所下降，尽管没有任何显著差异。此外，与NC组和Sham3组相比，LR3组中蓝藻显著富集，梭杆菌减少，而与LR3组相比，LR14组疣微菌门、绿弯菌门和脱铁杆菌门的丰度显著减少。厚壁菌门的丰度在LR3组中增加，在LR14组中再次下降。然而，拟杆菌门和放线菌门的丰度在LR3组中下降，在LR14组中再次增加。还揭示了肠道微生物群在属水平上的变化，急性肝损伤导致、、、UCG - 005和显著增加，以及和急剧下降。此外，基于Greengenes数据库，通过未观察状态重建的群落系统发育调查进行了功能宏基因组预测，揭示了信号转导、转录和细胞运动以及氨基酸、脂质、葡萄糖、辅因子和萜类化合物代谢以及外源性物质途径的改变。更好地了解50%PH诱导的急性肝损伤和修复后肠道微生物群的结构和功能变化，可能为肝切除术后宿主的恢复提供新的策略。
