Department of Biosphere Sciences, School of Human Sciences, Kobe College, 4-1, Okadayama, Nishinomiya, Hyogo, 662-8505, Japan.
Plant Cell Rep. 2012 Feb;31(2):291-8. doi: 10.1007/s00299-011-1163-2. Epub 2011 Oct 5.
Antioxidant activities of four flavonoids (rutin, quercetin, luteolin, and kaempferol) and two non-flavonoids (chlorogenic acid and pyrocatechol) against four reactive oxygen species (ROS) have been measured with a myoglobin method developed by our group. The myoglobin method uses the absorbance changes of myoglobin (a probe molecule) due to the reaction with the ROS as an indicator for the antioxidant activity measurement. Myoglobin protective ratio (MPR) was defined to express the antioxidant activities of the specimens. Antioxidant activities against hypochlorite ion, hydroxyl radical, peroxyl radical, and peroxynitrite were measured with the myoglobin method. The antioxidant activities were comprehensively evaluated by plotting MPR against four ROS and vitamin C equivalent concentration evaluated by DPPH quenching method in 5-axe cobweb charts. The four flavonoids show a very similar pattern in the 5-axe cobweb charts, while the patterns of two non-flavonoids are quite different from that of the flavonoids. This procedure combining the myoglobin method with the cobweb charts is useful in the evaluation of antioxidant activities of plant-derived food, and also can be extended to monitor antioxidant condition of media for plant cell cultures.
我们小组开发了一种肌红蛋白法，用于测量四种黄酮类化合物（芦丁、槲皮素、木樨草素和山奈酚）和两种非黄酮类化合物（绿原酸和邻苯二酚）对四种活性氧（ROS）的抗氧化活性。肌红蛋白法利用肌红蛋白（探针分子）与 ROS 反应引起的吸光度变化来指示抗氧化活性测量。肌红蛋白保护率（MPR）被定义为表达样品的抗氧化活性。用肌红蛋白法测量了对次氯酸根离子、羟基自由基、过氧自由基和过氧亚硝酸盐的抗氧化活性。通过在 5 轴蛛网图中绘制 MPR 与四种 ROS 的关系以及通过 DPPH 猝灭法评估的维生素 C 当量浓度，综合评估抗氧化活性。四种黄酮类化合物在 5 轴蛛网图中呈现出非常相似的模式，而两种非黄酮类化合物的模式与黄酮类化合物的模式有很大的不同。这种将肌红蛋白法与蛛网图相结合的方法可用于评估植物源性食品的抗氧化活性，也可扩展用于监测植物细胞培养介质的抗氧化状态。
