Division of Oral Diagnosis, Department of Diagnostic and Therapeutic Sciences, Meikai University School of Dentistry, Sakado, Saitama, Japan.
In Vivo. 2012 Nov-Dec;26(6):1015-20.
Occlusal raising method (so-called 'Template therapy') has been reported to alleviate various diseases and symptoms, but the underlying mechanism is not clear. We searched the low-molecular weight metabolite(s) in the saliva, the concentration of which is significantly changed by the template therapy.
One female patient with headache underwent the template therapy for 12 days, and her total saliva was subjected to non-targeted analysis using capillary electrophoresis time-of-flight mass spectrometry (CE-TOF-MS).
One hundred and thirteen substances were identified in the saliva. Glycine was the most abundant amino acid in the saliva, followed by alanine, serine and proline. After the start of the template therapy, her headache was alleviated, accompanied by a significant (p=0.042) increase of salivary concentration of glycine, as compared with total amino acids whereas that of other amino acids was not significantly changed. In the metabolomics profile, salivary concentration of large number of metabolites as compared with total metabolite concentration decreased, including N-acetylneuraminate (p=0.025) and p-hydroxyphenylacetate (p=0.039).
This pilot study demonstrated, to our knowledge for the first time, that only glycine exhibited unique changes among total metabolites, suggesting its significant role in template therapy.
咬合抬高法（所谓的“模板疗法”）已被报道可缓解各种疾病和症状，但其潜在机制尚不清楚。我们搜索了模板疗法显著改变的唾液中的低分子量代谢物（s）。
一名女性头痛患者接受模板治疗 12 天，对其总唾液进行毛细管电泳飞行时间质谱（CE-TOF-MS）的非靶向分析。
在唾液中鉴定出 113 种物质。甘氨酸是唾液中最丰富的氨基酸，其次是丙氨酸、丝氨酸和脯氨酸。模板治疗开始后，患者头痛缓解，与总氨基酸相比，甘氨酸的唾液浓度显著增加（p=0.042），而其他氨基酸的浓度没有明显变化。在代谢组学图谱中，与总代谢物浓度相比，大量代谢物的唾液浓度下降，包括 N-乙酰神经氨酸（p=0.025）和对羟基苯乙酸（p=0.039）。
本研究首次表明，模板治疗中只有甘氨酸在总代谢物中表现出独特的变化，提示其在模板治疗中具有重要作用。
