用于组织定向制造的层状丝支架。
Laminar silk scaffolds for aligned tissue fabrication.
机构信息
Department of Biotechnology, Indian Institute of Technology, Guwahati-781 039, India.
出版信息
Macromol Biosci. 2013 Jan;13(1):48-58. doi: 10.1002/mabi.201200230. Epub 2012 Nov 19.
Abstract
3D-biomaterial scaffolds with aligned architecture are of vital importance in tissue regeneration. A generic method is demonstrated to produce aligned biomaterial scaffolds using the physics of directional ice freezing. Homogeneously aligned 3D silk scaffolds with high porosity and alignment are prepared. The method can be adapted to a wide range of polymers and is devoid of any chemical reactions, thus avoiding potential complications associated with by-products. Mechanical properties and cellular responses with chondrocytes and bone-marrow-derived hMSCs are studied, assessing survival, proliferation, and differentiation. In vivo tests suggest biocompatibility of the matrices for future tissue engineering applications, specifically in areas where high cellular alignment is needed.
摘要
具有定向结构的 3D 生物材料支架在组织再生中具有重要意义。本文展示了一种通用方法，利用定向冰冻结的物理原理来制备定向生物材料支架。制备了具有高孔隙率和定向排列的均匀定向 3D 丝素支架。该方法可适用于多种聚合物，且不涉及任何化学反应，从而避免了与副产物相关的潜在并发症。研究了与软骨细胞和骨髓来源的 hMSCs 的机械性能和细胞反应，评估了细胞的存活率、增殖和分化。体内实验表明，该支架具有良好的生物相容性，可用于未来的组织工程应用，特别是在需要高细胞定向排列的区域。
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