BIOMAT, Dept. of Biological Sciences, Biotechnology Research Unit, Middle East Technical University, Ankara, Turkey.
Biomaterials. 2011 Aug;32(23):5320-9. doi: 10.1016/j.biomaterials.2011.04.025. Epub 2011 May 12.
The goal of this study was to design and develop a myocardial patch to use in the repair of myocardial infarctions or to slow down tissue damage and improve long-term heart function. The basic 3D construct design involved two biodegradable macroporous tubes, to allow transport of growth media to the cells within the construct, and cell seeded, aligned fiber mats wrapped around them. The microfibrous mat housed mesenchymal stem cells (MSCs) from human umbilical cord matrix (Wharton's Jelly) aligned in parallel to each other in a similar way to cell organization in native myocardium. Aligned micron-sized fiber mats were obtained by electrospinning a polyester blend (PHBV (5% HV), P(L-D,L)LA (70:30) and poly(glycerol sebacate) (PGS)). The micron-sized electrospun parallel fibers were effective in Wharton's Jelly (WJ) MSCs alignment and the cells were able to retract the mat. The 3D construct was cultured in a microbioreactor by perfusing the growth media transiently through the macroporous tubing for two weeks and examined by fluorescence microscopy for cell distribution and preservation of alignment. The fluorescence images of thin sections of 3D constructs from static and perfused cultures confirmed enhanced cell viability, uniform cell distribution and alignment due to nutrient provision from inside the 3D structure.
本研究的目的是设计和开发一种心肌补片，用于修复心肌梗死或减缓组织损伤并改善长期心脏功能。基本的 3D 结构设计涉及两个可生物降解的大孔管，以允许生长介质输送到构建物内的细胞，并在其周围包裹细胞接种、排列纤维垫。微孔纤维垫容纳人脐带基质（Wharton's Jelly）中的间充质干细胞（MSCs），以类似于天然心肌中细胞组织的方式彼此平行排列。通过静电纺丝聚酯共混物（PHBV（5%HV）、P（L-D，L）LA（70：30）和聚（甘油癸二酸酯）（PGS））获得平行排列的微尺寸纤维垫。微米级静电纺丝平行纤维有效促进了 Wharton's Jelly（WJ）MSCs 的排列，并且细胞能够缩回纤维垫。通过在大孔管中短暂灌注生长介质，将 3D 构建体在微生物反应器中培养两周，然后通过荧光显微镜检查细胞分布和排列的保存情况。静态和灌注培养的 3D 结构的薄片的荧光图像证实了由于 3D 结构内部提供营养物质，细胞活力增强、细胞分布均匀和排列一致。
