Kaminsky R, Gumm I D, Zweygarth E, Chuma F
International Laboratory for Research on Animal Diseases, Nairobi, Kenya.
Vet Parasitol. 1990 Jan;34(4):335-43. doi: 10.1016/0304-4017(90)90079-q.
Blood stream forms of drug-resistant and sensitive Trypanosoma brucei brucei, Trypanosoma brucei evansi and Trypanosoma vivax were incubated in a liquid medium for 24 h at 37 degrees C in the presence of various concentrations of diminazene aceturate (Berenil) or isometamidium chloride (Samorin), and assayed for infectivity in mice. Whereas the infectivity to mice of all Samorin-sensitive trypanosomes was decreased after incubation with 1 ng Samorin ml-1, the Samorin-resistant stocks remained infective for mice. Two of the Samorin-resistant stocks remained infective after incubation with Samorin concentrations of up to 50 ng ml-1. The infectivity of Berenil-resistant trypanosome stocks were also retained after incubation with drug concentrations (0.5 or 1.0 micrograms ml-1) which otherwise inhibited the infectivity of Berenil-sensitive trypanosome stocks. In addition, differences in infectivity were observed when Berenil-resistant and sensitive trypanosome stocks were incubated in medium supplemented with serum from goats previously treated with Berenil. Thus, drug-resistant and sensitive trypanosomes can be clearly distinguished using the drug incubation infectivity test.
将抗药性和敏感性布氏布氏锥虫、伊氏锥虫和活泼锥虫的血流形式在含有不同浓度的乙酰氨基苯脒（贝尼尔）或氯异脒（沙莫林）的液体培养基中于37℃孵育24小时，然后检测其对小鼠的感染性。与1纳克/毫升沙莫林孵育后，所有对沙莫林敏感的锥虫对小鼠的感染性均降低，而对沙莫林有抗性的虫株仍对小鼠具有感染性。其中两株对沙莫林有抗性的虫株在与高达50纳克/毫升的沙莫林孵育后仍具有感染性。与药物浓度（0.5或1.0微克/毫升）孵育后，贝尼尔抗性锥虫株的感染性也得以保留，而这些药物浓度会抑制贝尼尔敏感锥虫株的感染性。此外，当贝尼尔抗性和敏感锥虫株在补充有先前用贝尼尔处理过的山羊血清的培养基中孵育时，观察到了感染性的差异。因此，使用药物孵育感染性试验可以清楚地区分抗药性和敏感性锥虫。
