Gu Li, Zablocki Kyle, Lavelle Linda, Bodnar Stanko, Halperin Frederick, Harper Ike, Moghe Prabhas V, Uhrich Kathryn E
Rutgers, The State University of New Jersey, Department of Chemistry and Chemical Biology, 610 Taylor Road, Piscataway, New Jersey 08854, United States.
Polym Degrad Stab. 2012 Sep 1;97(9):1686-1689. doi: 10.1016/j.polymdegradstab.2012.06.017. Epub 2012 Jun 27.
An amphiphilic macromolecule (AM) was exposed to ionizing radiation (both electron beam and gamma) at doses of 25 kGy and 50 kGy to study the impact of these sterilization methods on the physicochemical properties and bioactivity of the AM. Proton nuclear magnetic resonance and gel permeation chromatography were used to determine the chemical structure and molecular weight, respectively. Size and zeta potential of the micelles formed from AMs in aqueous media were evaluated by dynamic light scattering. Bioactivity of irradiated AMs was evaluated by measuring inhibition of oxidized low-density lipoprotein uptake in macrophages. From these studies, no significant changes in the physicochemical properties or bioactivity were observed after the irradiation, demonstrating that the AMs can withstand typical radiation doses used to sterilize materials.
将一种两亲性大分子（AM）分别以25千戈瑞和50千戈瑞的剂量暴露于电离辐射（电子束和伽马射线）下，以研究这些灭菌方法对AM的物理化学性质和生物活性的影响。分别使用质子核磁共振和凝胶渗透色谱法来测定化学结构和分子量。通过动态光散射评估在水性介质中由AMs形成的胶束的尺寸和zeta电位。通过测量对巨噬细胞中氧化型低密度脂蛋白摄取的抑制作用来评估辐照后AMs的生物活性。从这些研究中可以看出，辐照后未观察到物理化学性质或生物活性有显著变化，这表明AMs能够承受用于材料灭菌的典型辐射剂量。
