Monti Filippo, Manfredi Giovanni, Palamà Ilaria Elena, Kovtun Alessandro, Zangoli Mattia, D'Amone Stefania, Ortolani Luca, Bondelli Gaia, Szreder Tomasz, Bobrowski Krzysztof, D'Angelantonio Mila, Lanzani Guglielmo, Di Maria Francesca
Consiglio Nazionale delle Ricerche, CNR-ISOF, via P. Gobetti 101, Bologna, 40129, Italy.
Center for Nano Science and Technology, Istituto Italiano di Tecnologia, via Pascoli 70/3, Milano, 20133, Italy.
Adv Healthc Mater. 2021 Mar;10(6):e2001306. doi: 10.1002/adhm.202001306. Epub 2021 Jan 14.
In this work, the feasibility of sterilizing a water suspension of poly-3-hexylthiophene nanoparticles (P3HT-NPs) is investigated using ionizing radiation, either γ-rays or high-energy electrons (e-beam). It is found that regardless of the irradiation source, the size, polydispersity, aggregation stability, and morphology of the NPs are not affected by the treatment. Furthermore, the impact of ionizing radiation on the physicochemical properties of NPs at different absorbed radiation doses (10-25 kGy) and dose rates (kGy time ) is evaluated through different spectroscopic techniques. The results indicate that delivering a high dose of radiations (25 kGy) at a high dose rate, that is, kGy s , as achieved by e-beam irradiation, preserves the characteristics of the polymeric NPs. Differently, the same radiation dose but delivered at a lower dose rate, that is, kGy h , as attained by using a γ-source, can modify the physicochemical properties of the polymer. Sterility tests indicate that an absorbed dose of 10 kGy, delivered either with γ-rays or e-beam, is already sufficient for effective sterilization of the colloidal suspension and for reducing the endotoxin content. Finally, NPs irradiated at different doses, exhibit the same cytocompatibility and cell internalization characteristics in human neuroblastoma SH-SY5Y cells of NPs prepared under aseptic conditions.
在这项工作中，研究了使用电离辐射（γ射线或高能电子束）对聚3-己基噻吩纳米颗粒（P3HT-NPs）水悬浮液进行灭菌的可行性。结果发现，无论辐照源如何，纳米颗粒的尺寸、多分散性、聚集稳定性和形态均不受该处理的影响。此外，通过不同的光谱技术评估了电离辐射在不同吸收辐射剂量（10-25 kGy）和剂量率（kGy/时间）下对纳米颗粒物理化学性质的影响。结果表明，如电子束辐照所实现的那样，以高剂量率（即kGy/s）施加高剂量辐射（25 kGy），可保留聚合物纳米颗粒的特性。不同的是，相同的辐射剂量但以较低剂量率（即kGy/h）施加，如使用γ源所达到的那样，会改变聚合物的物理化学性质。无菌测试表明，以γ射线或电子束施加10 kGy的吸收剂量，已足以有效灭菌胶体悬浮液并降低内毒素含量。最后，在不同剂量下辐照的纳米颗粒，在无菌条件下制备的纳米颗粒在人神经母细胞瘤SH-SY5Y细胞中表现出相同的细胞相容性和细胞内化特性。
