Esmaeili Akbar, Khakpoor Marjan
Department of Chemical Engineering, North Tehran Branch, Islamic Azad University, Tehran, Iran.
Acta Biochim Pol. 2012;59(4):581-5. Epub 2012 Nov 16.
The objective of this study was to evaluate the chemical composition CH3OH-CH2Cl2 (1:1) extract and biological activities of various extracts derived from the aerial parts of the brown marine alga Stoechospermum marginatum (C. Agardh). Gas chromatography (GC) and gas chromatography-mass spectroscopy (GC-MS) were used to analyze the composition of the essential oil. Total phenolics assay demonstrated a high value in hexane extract (HE), with a lower value for chloroform extract (CE), and the lowest value for methanol extract (ME). DPPH (2,2-diphenyl-1-picrylhydrazyl) assay showed that extracts of S. marginatum possess radical scavenging activity (RSA). Tests of the antioxidant property of the extracts revealed both electron and hydrogen transfer mechanisms. The antibacterial activity of the ME, CE, and HE as well as an ethanol extract was estimated against seven Gram-positive and Gram-negative bacteria. The ethanol extract showed the highest antibacterial activity, and the HE showed the lowest.
本研究的目的是评估棕色海藻缘管浒苔（C. 阿加德）地上部分的CH3OH-CH2Cl2（1:1）提取物的化学成分以及各种提取物的生物活性。采用气相色谱（GC）和气相色谱-质谱联用（GC-MS）分析精油成分。总酚含量测定表明，己烷提取物（HE）中的含量较高，氯仿提取物（CE）中的含量较低，甲醇提取物（ME）中的含量最低。二苯基苦味酰基自由基（DPPH）测定表明，缘管浒苔提取物具有自由基清除活性（RSA）。提取物抗氧化性能测试揭示了电子和氢转移机制。评估了ME、CE、HE以及乙醇提取物对7种革兰氏阳性和革兰氏阴性细菌的抗菌活性。乙醇提取物显示出最高的抗菌活性，而HE显示出最低的抗菌活性。
