Department of Chemistry, Mass Spectrometry Centre, LAQV REQUIMTE, University of Aveiro, Santiago University Campus, 3810-193, Aveiro, Portugal.
Department of Chemistry, CESAM-Centre for Environmental and Marine Studies, University of Aveiro, Santiago University Campus, 3810-193, Aveiro, Portugal.
Sci Rep. 2021 Feb 23;11(1):4355. doi: 10.1038/s41598-021-83455-y.
There is a growing trend to explore microalgae as an alternative resource for the food, feed, pharmaceutical, cosmetic and fuel industry. Moreover, the polar lipidome of microalgae is interesting because of the reports of bioactive polar lipids which could foster new applications for microalgae. In this work, we identified for the first time the Chlorococcum amblystomatis lipidome using hydrophilic interaction liquid chromatography-high resolution electrospray ionization- tandem mass spectrometry (HILIC-HR-ESI-MS/MS). The Chlorococcum amblystomatis strain had a lipid content of 20.77% and the fatty acid profile, determined by gas chromatography-mass spectrometry, has shown that this microalga contains high amounts of omega-3 polyunsaturated fatty acids (PUFAs). The lipidome identified included 245 molecular ions and 350 lipid species comprising 15 different classes of glycolipids (6), phospholipids (7) and betaine lipids (2). Of these, 157 lipid species and the main lipid species of each class were esterified with omega-3 PUFAs. The lipid extract has shown antioxidant activity and anti-inflammatory potential. Lipid extracts also had low values of atherogenic (0.54) and thrombogenic index (0.27). In conclusion, the lipid extracts of Chlorococcum amblystomatis have been found to be a source of lipids rich in omega-3 PUFAs for of great value for the food, feed, cosmetic, nutraceutical and pharmaceutical industries.
越来越多的人开始探索微藻作为食品、饲料、制药、化妆品和燃料行业的替代资源。此外，由于报道称生物活性极性脂类可以为微藻开拓新的应用领域，因此微藻的极性脂类组学也很有趣。在这项工作中，我们首次使用亲水相互作用液相色谱-高分辨电喷雾电离-串联质谱法（HILIC-HR-ESI-MS/MS）对钝顶螺旋藻的脂类组学进行了鉴定。钝顶螺旋藻的脂类含量为 20.77%，通过气相色谱-质谱法确定的脂肪酸图谱表明，这种微藻含有大量的ω-3 多不饱和脂肪酸（PUFAs）。鉴定出的脂类组包括 245 个分子离子和 350 种脂类，包括 15 种不同类别的糖脂（6）、磷脂（7）和甜菜碱脂（2）。其中，157 种脂类和每种类别的主要脂类都与 ω-3 PUFAs 酯化。脂质提取物具有抗氧化和抗炎潜力。脂质提取物的致动脉粥样硬化指数（0.54）和血栓形成指数（0.27）也较低。总之，发现钝顶螺旋藻的脂质提取物是富含 ω-3 PUFAs 的脂质的来源，对食品、饲料、化妆品、营养保健品和制药行业具有重要价值。
