Chen Yan-yan, Qian Da-wei, Shang Er-xin, Qian Ye-fei, Liu Pei, Su Shu-lan, Guo Jian-ming, Tang Yu-ping, Duan Jin-ao
Jiangsu Key Laboratory for High Technology Research of Traditional Chinese Medicine Formulae, Nanjing University of Chinese Medicine, Nanjing 210046, China.
Yao Xue Xue Bao. 2012 Aug;47(8):1043-8.
By investigating the interaction between components from Flos Genkwa (FG) and Radix et Rhizoma Glycyrrhizae (RRG) and the dissolution profile of toxic components in co-decoction, the characteristics and possible mechanism of incompatibility were revealed. Ultra-high performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) and ultra-high performance liquid chromatography triple-quadrupole mass spectrometry (UPLC-TQ/MS) were used to analyze multi-components in different herb extractions prepared by different ratios of FG/FG processed by vinegar (FGV) and RRG, which reflect the interaction and characteristics of multiple components in incompatibility combinations. The results showed that the components dissolution was influenced by compatibility ratio with certain regularity. Whether FG processed by vinegar or not, with the increase of RRG in co-decoction, the dissolution of diterpenes, especially for yuanhuacine, yuanhuadine and yuanhuajine, the toxic ingredients of FG, increased significantly. From these results, the material basis and one possible mechanism of incompatibility between FG and RRG is the increasing dissolution of diterpenes, toxic components of FG in co-decoction process, which caused by interaction between multi-components in these two herbs.
通过研究芫花（FG）与甘草（RRG）各组分之间的相互作用以及共煎液中有毒成分的溶出情况，揭示了“十八反”的特点及可能机制。采用超高效液相色谱四极杆飞行时间质谱（UPLC-Q-TOF/MS）和超高效液相色谱三重四极杆质谱（UPLC-TQ/MS）分析不同比例醋制芫花（FGV）与RRG制备的不同药对提取物中的多成分，以反映“十八反”配伍组合中多成分的相互作用及特征。结果表明，成分溶出受配伍比例影响，具有一定规律性。无论芫花是否醋制，共煎液中随着RRG比例增加，芫花二萜类成分尤其是芫花酯甲、芫花酯丁和芫花萜（芫花的毒性成分）的溶出显著增加。由此可知，FG与RRG“十八反”的物质基础及一种可能机制是共煎过程中FG的毒性成分二萜类成分溶出增加，这是两味药中多成分相互作用所致。
