Zhang Shixuan, Zhang Fenghong, Li Xiaona, Dong Weiqun, Wen Luohan, Wang Shisheng
State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116012, People's Republic of China.
Phytochem Anal. 2007 Mar-Apr;18(2):91-7. doi: 10.1002/pca.953.
Daphne genkwa contains a novel class of anticancer diterpene esters that inhibit DNA topoisomerase I. Fingerprint and quantitative analysis by HPLC were performed in order to characterise and evaluate D. genkwa. A standard fingerprint of Daphne diterpene esters from the root extract was first established by HPLC-UV, and the major peaks in the fingerprint profile were preliminarily determined using HPLC-MS. The principal Daphne diterpene esters, yuanhuacine (1), yuanhuadine (2), yuanhuajine (3) and yuanhuagine (4), were isolated and identified using a combination of UV, IR, MS, 1H-NMR and 13C-NMR spectral data. Quantitative analysis indicated that 1 was the principal component in the root, and that 2 was the major component in the buds. The average extraction rates of 1 and 2 were 0.0151 and 0.0033% (n=10) from the root, respectively, and 0.0020 and 0.0078% (n=3) from the buds, respectively.
芫花含有一类新型的抗癌二萜酯，可抑制DNA拓扑异构酶I。为了对芫花进行表征和评估，采用高效液相色谱法（HPLC）进行指纹图谱和定量分析。首先通过HPLC-UV建立了根提取物中芫花二萜酯的标准指纹图谱，并使用HPLC-MS初步确定了指纹图谱中的主要峰。采用紫外光谱（UV）、红外光谱（IR）、质谱（MS）、核磁共振氢谱（1H-NMR）和核磁共振碳谱（13C-NMR）光谱数据相结合的方法，分离并鉴定了主要的芫花二萜酯，即芫花酯甲（1）、芫花酯丁（2）、芫花酯丙（3）和芫花酯戊（4）。定量分析表明，1是根中的主要成分，2是芽中的主要成分。1和2从根中的平均提取率分别为0.0151%和0.0033%（n=10），从芽中的平均提取率分别为0.0020%和0.0078%（n=3）。
