Gutiérrez M C, Castillo A M, Pagaling E, Heaphy S, Kamekura M, Xue Y, Ma Y, Cowan D A, Jones B E, Grant W D, Ventosa A
Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla, 41012 Sevilla, Spain.
Int J Syst Evol Microbiol. 2008 Sep;58(Pt 9):2031-5. doi: 10.1099/ijs.0.65840-0.
A Gram-negative, non-motile, neutrophilic, rod-shaped, extremely halophilic archaeon, designated strain BG-1(T), was isolated from a salt lake, Lake Bagaejinnor, in Inner Mongolia, China. Strain BG-1(T) was able to grow at 25-55 degrees C, required at least 2.5 M NaCl for growth (with an optimum at 3.4 M NaCl) and grew at pH 6.0-9.0 (with an optimum at pH 7.5). Hypotonic treatment with less than 2.0 M NaCl caused cell lysis. Phylogenetic analysis of the almost-complete 16S rRNA gene sequence positioned the isolate within the genus Halorubrum in the family Halobacteriaceae. Strain BG-1(T) was most closely related to Halorubrum aidingense 31-hong(T) (98.8% sequence similarity), Halorubrum saccharovorum NCIMB 2081(T) (98.6%), Halorubrum lacusprofundi ACAM 34(T) (98.6%) and Halorubrum lipolyticum 9-3(T) (98.4%). However, values for DNA-DNA hybridization between strain BG-1(T) and the most closely related members of the genus Halorubrum were below 40%. Analysis of the polar lipids of strain BG-1(T) revealed the presence of mannosyl-2-sulfate-(1-4)-glycosyl-archaeol, the main glycolipid found in neutrophilic species of the genus Halorubrum. The G+C content of the genomic DNA was 69.4 mol% (T(m)). Comparison of the phenotypic characteristics of the strain with those of Halorubrum species supported the conclusion that BG-1(T) represents a novel species within this genus, for which the name Halorubrum kocurii sp. nov. is proposed. The type strain is BG-1(T) (=CECT 7322(T) =CGMCC 1.7018(T) =JCM 14978(T)).
从中国内蒙古巴嘎淖尔盐湖分离出一株革兰氏阴性、无运动性、嗜中性、杆状、极端嗜盐古菌，命名为菌株BG-1(T)。菌株BG-1(T)能够在25至55摄氏度生长，生长需要至少2.5 M NaCl（最适浓度为3.4 M NaCl），在pH 6.0至9.0生长（最适pH为7.5）。用低于2.0 M NaCl进行低渗处理会导致细胞裂解。对近乎完整的16S rRNA基因序列进行系统发育分析，将该分离株置于嗜盐菌科嗜盐红菌属内。菌株BG-1(T)与嗜盐辅助红菌31-hong(T)（序列相似性98.8%）、嗜糖嗜盐红菌NCIMB 2081(T)（98.6%）、深湖嗜盐红菌ACAM 34(T)（98.6%）和嗜脂嗜盐红菌9-3(T)（98.4%）关系最为密切。然而，菌株BG-1(T)与嗜盐红菌属关系最密切的成员之间的DNA-DNA杂交值低于40%。对菌株BG-1(T)的极性脂质分析表明存在甘露糖基-2-硫酸盐-(1-4)-糖基-古菌醇，这是嗜盐红菌属嗜中性物种中发现的主要糖脂。基因组DNA的G+C含量为69.4 mol%（熔解温度）。将该菌株与嗜盐红菌属物种的表型特征进行比较，支持了BG-1(T)代表该属内一个新物种的结论，为此提出新名称嗜盐科库里红菌（Halorubrum kocurii sp. nov.）。模式菌株为BG-1(T)（=CECT 7322(T)=CGMCC 1.7018(T)=JCM 14978(T)）。
