Strand S E, Persson B R
J Nucl Med. 1979 Oct;20(10):1038-46.
The activity-size distribution of radiocolloids has been studied using gel-chromatography scanning (GCS) of columns filled with Sepharose 4B gel. Rabbits were injected subcutaneously with the colloid of interest, laid supine beneath a gamma camera, and imaged every 15 sec for 2 to 4 hr. From the stored data, the uptakes in the parasternal lymph nodes were analyzed in terms of two-compartment model, and the rate constants measured. The substances tested were Au- 198 colloid, Tc-99m antimony sulfide colloid, Tc-99m tin colloid, Tc-99m phytate, and Tc-99m sulfur colloid. It was shown that the optimal particle size for the colloid in the range 1-10 nm. The large and most rapid uptake was found for Au- 198 colloid, with a particle size of 5 nm, which appeared as a single peak in the GCS spectrum. The percentage uptake after 2 hr for Au- 198 colloid 8%, while it was 5% for antimony-sulfide colloid, which was the best of the Tc-99m-labeled colloids. The GCS spectrum for the antimony product showed a single-peaked size distribution with a somewhat broader range: 5-15 nm. The particles of the other colloids were either too large to pass into the lymphatic system, or too small to be trapped.
已使用填充有琼脂糖4B凝胶的柱的凝胶色谱扫描（GCS）研究了放射性胶体的活度-大小分布。给兔子皮下注射感兴趣的胶体，使其仰卧在γ相机下方，并每隔15秒成像2至4小时。根据存储的数据，按照双室模型分析胸骨旁淋巴结的摄取情况，并测量速率常数。测试的物质有Au-198胶体、Tc-99m硫化锑胶体、Tc-99m锡胶体、Tc-99m植酸盐和Tc-99m硫胶体。结果表明，胶体的最佳粒径范围为1-10nm。发现粒径为5nm的Au-198胶体摄取量大且速度最快，在GCS光谱中呈现为单峰。Au-198胶体2小时后的摄取百分比为8%，而硫化锑胶体为5%，硫化锑胶体是Tc-99m标记胶体中摄取效果最好的。锑产品的GCS光谱显示单峰大小分布，范围稍宽：5-15nm。其他胶体的颗粒要么太大无法进入淋巴系统，要么太小无法被捕获。
