人红细胞中的淀粉样β水平。
Amyloid β levels in human red blood cells.
机构信息
Food and Biodynamic Chemistry Laboratory, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
出版信息
PLoS One. 2012;7(11):e49620. doi: 10.1371/journal.pone.0049620. Epub 2012 Nov 15.
UNLABELLED
Amyloid β-peptide (Aβ) is hypothesized to play a key role by oxidatively impairing the capacity of red blood cells (RBCs) to deliver oxygen to the brain. These processes are implicated in the pathogenesis of Alzheimer's disease (AD). Although plasma Aβ has been investigated thoroughly, the presence and distribution of Aβ in human RBCs are still unclear. In this study, we quantitated Aβ40 and Aβ42 in human RBCs with ELISA assays, and provided evidence that significant amounts of Aβ could be detected in RBCs and that the RBC Aβ levels increased with aging. The RBC Aβ levels increased with aging. On the other hand, providing an antioxidant supplement (astaxanthin, a polar carotenoid) to humans was found to decrease RBC Aβ as well as oxidative stress marker levels. These results suggest that plasma Aβ40 and Aβ42 bind to RBCs (possibly with aging), implying a pathogenic role of RBC Aβ. Moreover, the data indicate that RBC Aβ40 and Aβ42 may constitute biomarkers of AD. As a preventive strategy, therapeutic application of astaxanthin as an Aβ-lowering agent in RBCs could be considered as a possible anti-dementia agent.
TRIAL REGISTRATION
Controlled-Trials.com ISRCTN42483402.
未注明
淀粉样β肽（Aβ）被认为通过氧化作用损害红细胞（RBC）向大脑输送氧气的能力，从而在阿尔茨海默病（AD）的发病机制中起关键作用。虽然已经对血浆 Aβ 进行了深入研究，但 Aβ 在人 RBC 中的存在和分布仍不清楚。在这项研究中，我们用 ELISA 检测法定量了人 RBC 中的 Aβ40 和 Aβ42，并提供了证据表明可以在 RBC 中检测到大量的 Aβ，并且 RBC Aβ 水平随年龄增长而增加。另一方面，给人类提供抗氧化补充剂（虾青素，一种极性类胡萝卜素）被发现可以降低 RBC Aβ 以及氧化应激标志物的水平。这些结果表明，血浆 Aβ40 和 Aβ42 与 RBC 结合（可能与年龄有关），暗示 RBC Aβ 具有致病性。此外，数据表明 RBC Aβ40 和 Aβ42 可能是 AD 的生物标志物。作为一种预防策略，虾青素作为 RBC 中 Aβ 降低剂的治疗应用可以被认为是一种可能的抗痴呆药物。
试验注册
受控试验.com ISRCTN42483402。
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