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伯克霍尔德氏菌 MBA4 中乙酸盐和卤乙酸盐的转运由不同的系统操作。

Transports of acetate and haloacetate in Burkholderia species MBA4 are operated by distinct systems.

机构信息

Molecular Microbiology Laboratory, School of Biological Sciences, The University of Hong Kong, Pokfulam Road, Hong Kong, Hong Kong.

出版信息

BMC Microbiol. 2012 Nov 20;12:267. doi: 10.1186/1471-2180-12-267.

DOI:10.1186/1471-2180-12-267
PMID:23167477
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3552994/
Abstract

BACKGROUND

Acetate is a commonly used substrate for biosynthesis while monochloroacetate is a structurally similar compound but toxic and inhibits cell metabolism by blocking the citric acid cycle. In Burkholderia species MBA4 haloacetate was utilized as a carbon and energy source for growth. The degradation of haloacid was mediated by the production of an inducible dehalogenase. Recent studies have identified the presence of a concomitantly induced haloacetate-uptake activity in MBA4. This uptake activity has also been found to transport acetate. Since acetate transporters are commonly found in bacteria it is likely that haloacetate was transported by such a system in MBA4.

RESULTS

The haloacetate-uptake activity of MBA4 was found to be induced by monochloroacetate (MCA) and monobromoacetate (MBA). While the acetate-uptake activity was also induced by MCA and MBA, other alkanoates: acetate, propionate and 2-monochloropropionate (2MCPA) were also inducers. Competing solute analysis showed that acetate and propionate interrupted the acetate- and MCA- induced acetate-uptake activities. While MCA, MBA, 2MCPA, and butyrate have no effect on acetate uptake they could significantly quenched the MCA-induced MCA-uptake activity. Transmembrane electrochemical potential was shown to be a driving force for both acetate- and MCA- transport systems.

CONCLUSIONS

Here we showed that acetate- and MCA- uptake in Burkholderia species MBA4 are two transport systems that have different induction patterns and substrate specificities. It is envisaged that the shapes and the three dimensional structures of the solutes determine their recognition or exclusion by the two transport systems.

摘要

背景

乙酸盐是生物合成中常用的底物，而一氯乙酸盐是一种结构相似的化合物，但有毒，通过阻断柠檬酸循环来抑制细胞代谢。在伯克霍尔德氏菌属 MBA4 中，卤代乙酸盐被用作生长的碳源和能源。卤代酸的降解是通过诱导脱卤酶的产生来介导的。最近的研究表明，在 MBA4 中存在同时诱导的卤代乙酸摄取活性。这种摄取活性也被发现可以转运乙酸盐。由于乙酸盐转运体在细菌中很常见，因此卤代乙酸盐很可能是通过 MBA4 中的这种系统进行转运的。

结果

发现 MBA4 的卤代乙酸盐摄取活性可被一氯乙酸（MCA）和单溴乙酸（MBA）诱导。虽然乙酸盐摄取活性也可被 MCA 和 MBA 诱导，但其他烷酸盐：乙酸盐、丙酸盐和 2-单氯丙酸盐（2MCPA）也是诱导物。竞争溶质分析表明，乙酸盐和丙酸盐中断了乙酸盐和 MCA 诱导的乙酸盐摄取活性。虽然 MCA、MBA、2MCPA 和丁酸盐对乙酸盐摄取没有影响，但它们可以显著抑制 MCA 诱导的 MCA 摄取活性。跨膜电化学势被证明是乙酸盐和 MCA 转运系统的驱动力。

结论

在这里，我们表明 Burkholderia 属 MBA4 中的乙酸盐和 MCA 摄取是两种具有不同诱导模式和底物特异性的转运系统。可以预见，溶质的形状和三维结构决定了它们被两种转运系统的识别或排斥。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f14/3552994/e1306befaff2/1471-2180-12-267-7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f14/3552994/bee7ead54d69/1471-2180-12-267-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f14/3552994/9ae1e9b3ebc6/1471-2180-12-267-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f14/3552994/d43ba3485a9a/1471-2180-12-267-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f14/3552994/dbb1995fd34b/1471-2180-12-267-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f14/3552994/75799cede72b/1471-2180-12-267-5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f14/3552994/b875fcf816ac/1471-2180-12-267-6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f14/3552994/e1306befaff2/1471-2180-12-267-7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f14/3552994/bee7ead54d69/1471-2180-12-267-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f14/3552994/9ae1e9b3ebc6/1471-2180-12-267-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f14/3552994/d43ba3485a9a/1471-2180-12-267-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f14/3552994/dbb1995fd34b/1471-2180-12-267-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f14/3552994/75799cede72b/1471-2180-12-267-5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f14/3552994/b875fcf816ac/1471-2180-12-267-6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f14/3552994/e1306befaff2/1471-2180-12-267-7.jpg

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Fructose increases the resistance of Rhodobacter capsulatus to the toxic oxyanion tellurite through repression of acetate permease (ActP).果糖通过抑制乙酸盐透性酶（ActP）增加荚膜红细菌对有毒氧代阴离子碲酸盐的抗性。
Antonie Van Leeuwenhoek. 2011 Nov;100(4):655-8. doi: 10.1007/s10482-011-9619-5. Epub 2011 Jul 7.
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Haloacetic acids in swimming pools: swimmer and worker exposure.
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Environ Sci Technol. 2011 Jul 1;45(13):5783-90. doi: 10.1021/es103959d. Epub 2011 Jun 7.
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Mammalian cell cytotoxicity and genotoxicity of the haloacetic acids, a major class of drinking water disinfection by-products.卤乙酸类消毒副产物是饮用水主要消毒副产物之一，该物质对哺乳动物细胞的细胞毒性和遗传毒性。
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Topological analysis of a haloacid permease of a Burkholderia sp. bacterium with a PhoA-LacZ reporter.利用PhoA-LacZ报告基因对伯克霍尔德氏菌属细菌的卤代酸通透酶进行拓扑分析。
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