Han Seung-Hee, Chung Tae-Ho, Nam Eui-Hwa, Park Seol-Hee, Hwang Cheol-Yong
College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 151-742, South Korea.
Med Mycol. 2013 May;51(4):396-404. doi: 10.3109/13693786.2012.740575. Epub 2012 Nov 21.
We investigated Malassezia species and genotypes colonizing dogs, comparing those recovered from the ear canal with those from other anatomical body sites, as well as from diseased and healthy skin. The Malassezia isolates were obtained from four types of skin samples, i.e., diseased ear, diseased skin, healthy ear, and healthy skin. Sequences of the 26S ribosomal DNA region, the intergenic spacer 1 (IGS-1) and the internal transcribed spacer 1 (ITS-1) DNA region were analyzed. These confirmed the presence of Malassezia pachydermatis, which could be separated into three main sequence genotype groups (A, B, C). Genotype A was the most common, only two genotype B isolates were recovered from diseased skin lesion and genotype C was more likely to be isolated from ear samples than from other healthy or diseased-skin sites. The present findings provide the basis for further studies of genotypic diversity in M. pachydermatis, as well as their pathogenic potential.
我们调查了定植于犬类的马拉色菌属菌种和基因型，比较了从耳道分离出的菌株与从其他身体解剖部位以及患病和健康皮肤分离出的菌株。马拉色菌分离株取自四种类型的皮肤样本，即患病耳部、患病皮肤、健康耳部和健康皮肤。对26S核糖体DNA区域、基因间隔区1（IGS-1）和内部转录间隔区1（ITS-1）DNA区域的序列进行了分析。这些分析证实了厚皮马拉色菌的存在，其可分为三个主要序列基因型组（A、B、C）。基因型A最为常见，仅从患病皮肤损伤处分离出两株基因型B的菌株，基因型C从耳部样本中分离出来的可能性高于从其他健康或患病皮肤部位。目前的研究结果为进一步研究厚皮马拉色菌的基因型多样性及其致病潜力提供了依据。
