Kobayashi Tetsuya, Kano Rui, Nagata Masahiko, Hasegawa Atsuhiko, Kamata Hiroshi
Department of Pathobiology, Nihon University School of Veterinary Medicine, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan.
Vet Dermatol. 2011 Oct;22(5):401-5. doi: 10.1111/j.1365-3164.2011.00961.x. Epub 2011 Mar 14.
Isolates of Malassezia pachydermatis from healthy dog skin and from dogs with atopic dermatitis were molecularly characterized using internal spacer 1 (IGS1) region analyses, and their phospholipase A2 activity and pH growth profiles were then characterized in vitro. The percentage of isolates from healthy dogs that had the following IGS1 subtypes (isotype, %) were as follows: 1A, 6%; 1B, 27%; 1C, 11%; 2A, 6%; 2B, 6%; 3A, 11%; 3C, 3%; and 3D, 24%. In contrast, 9% of isolates from dogs with atopic dermatitis were isotype IB and 91% were isotype 3D, indicating that isolates of subtype 3D were the most prevalent in dogs with atopic dermatitis. Production of phospholipase A2 was statistically higher in isolates of subtype 3D than in the other subtypes. The subtype 3D isolates showed enhanced growth on alkaline medium compared with non-3D subtype isolates. The main clinical sign of canine Malassezia dermatitis is waxy exudates on the skin, which predispose the patient to development of a yeast overgrowth of the subtype 3D. Increased phospholipase A2 production may be involved in the inflammatory process associated with Malassezia dermatitis.
利用内部间隔区1（IGS1）区域分析对从健康犬皮肤和患有特应性皮炎的犬身上分离出的厚皮马拉色菌进行分子特征分析，然后在体外对其磷脂酶A2活性和pH生长曲线进行特征分析。来自健康犬的分离株中具有以下IGS1亚型（同型，%）的百分比情况如下：1A，6%；1B，27%；1C，11%；2A，6%；2B，6%；3A，11%；3C，3%；以及3D，24%。相比之下，来自患有特应性皮炎的犬的分离株中9%为同型IB，91%为同型3D，这表明3D亚型的分离株在患有特应性皮炎的犬中最为普遍。3D亚型分离株的磷脂酶A2产量在统计学上高于其他亚型。与非3D亚型分离株相比，3D亚型分离株在碱性培养基上生长增强。犬马拉色菌性皮炎的主要临床症状是皮肤上有蜡样渗出物，这使患者易发生3D亚型酵母菌过度生长。磷脂酶A2产量增加可能与马拉色菌性皮炎相关的炎症过程有关。
