Zou Bo, Ji Ping, Sun Shoujuan, Qi Xiaojuan
Dept. of Oral and Maxillofacial Surgery, Hospital of Stomatology, Chongqing Medical University, Chongqing Research Center for Oral Diseases and Biomedical Science, Chongqing 401147, China.
Hua Xi Kou Qiang Yi Xue Za Zhi. 2012 Oct;30(5):458-62.
To screen the differentially expressed genes of stem-like cells in tongue squamous cell carcinoma using suppression subtractive hybridization (SSH).
The cells with high and low expression of aldehyde dehydrogenase(ALDHbr and ALDHlow) were isolated respectively from human tongue squamous cell carcinoma Tca8113 cell line. SSH was used to identify differentially expressed genes in those two groups. Positive cell clones with high/ low-expressed ALDH were screened randomly for the genetic sequences and function analysis by the bioinformatics technology.
Total 62 differentially expressed genes were identified, most of which got involved in regulation of cell cycle, cellular differentiation and other biology activities as gene ontology (GO) analysis indicated. Signal pathway analysis revealed 12 pathways related to 11 genes changed significantly.
Several potential novel biomarker candidates of tongue cancer stem cells were identified and they may provide important insights into the functions and characteristics of human tumor stem cell.
运用抑制性消减杂交技术（SSH）筛选舌鳞状细胞癌中干细胞的差异表达基因。
分别从人舌鳞状细胞癌Tca8113细胞系中分离出醛脱氢酶高表达（ALDHbr）和低表达（ALDHlow）的细胞。运用SSH技术鉴定这两组细胞中的差异表达基因。随机筛选出高/低表达ALDH的阳性细胞克隆，采用生物信息学技术进行基因序列和功能分析。
共鉴定出62个差异表达基因，基因本体（GO）分析表明，其中大部分基因参与细胞周期调控、细胞分化及其他生物学活动。信号通路分析显示，11个基因发生显著变化，涉及12条信号通路。
鉴定出了几种潜在的舌癌干细胞新型生物标志物候选基因，它们可能为深入了解人类肿瘤干细胞的功能和特性提供重要线索。
