Wang Jun, Li Lifeng, Gao Lina, Guan Chao, Su Kexin, Li Linlin, Luo Wenping, Chen Hongying, Ji Ping
Department of Periodontal Surgery, College of Stomatology, Chongqing Medical University, Chongqing 401147, P.R. China.
Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing 401147, P.R. China.
Oncol Lett. 2017 Dec;14(6):7055-7068. doi: 10.3892/ol.2017.7108. Epub 2017 Sep 29.
Previous studies have demonstrated that cancer cells with increased levels of aldehyde dehydrogenase 'bright' activity (ALDH) exhibit stem cell properties compared with cells exhibiting decreased ALDH activity (ALDH). To screen possible biomarkers of cancer stem cells in tongue squamous cell carcinoma, ALDH and ALDH cells were isolated from the tongue squamous cell carcinoma TCA8113 cell line, and suppression subtractive hybridization was performed to identify differentially expressed genes in the two subpopulations. A total of 240 positive clones were randomly selected for sequencing and were functionally characterized using bioinformatical tools. The results of the present study identified the differential expression of 104 clones, 62 of which corresponded to known genes and 42 of which corresponded to unknown genes. Cluster analysis revealed that the known genes were involved in the regulation of the cell cycle and cell differentiation. In addition, analysis of 10 signaling pathways revealed that genes were markedly altered in the ALDH cell subpopulation. Additional study is required to identify the function that these genes serve in the biomolecular regulatory mechanisms of cancer stem cells and to assist in explaining the biological behavior of oral squamous cell carcinoma.
先前的研究表明，与醛脱氢酶活性降低（ALDH）的细胞相比，醛脱氢酶“明亮”活性（ALDH）水平升高的癌细胞表现出干细胞特性。为了筛选舌鳞状细胞癌中癌症干细胞的潜在生物标志物，从舌鳞状细胞癌TCA8113细胞系中分离出ALDH和ALDH细胞，并进行抑制性消减杂交以鉴定这两个亚群中差异表达的基因。总共随机选择240个阳性克隆进行测序，并使用生物信息学工具对其进行功能表征。本研究结果鉴定出104个克隆的差异表达，其中62个对应已知基因，42个对应未知基因。聚类分析显示，已知基因参与细胞周期和细胞分化的调控。此外，对10条信号通路的分析表明，基因在ALDH细胞亚群中发生了明显改变。需要进一步研究以确定这些基因在癌症干细胞生物分子调控机制中所起的作用，并有助于解释口腔鳞状细胞癌的生物学行为。
