Yang Jing, Ji Ping, Zou Bo, Sun Shoujuan, Qi Xiaojuan
Dept. of Oral and Maxillofacial Surgery, The Affiliated Hospital of Stomatology, Chongqing Medical University, Chongqing 401147, China.
Hua Xi Kou Qiang Yi Xue Za Zhi. 2012 Aug;30(4):439-43.
To detect the expression of aldehyde dehydrogenase (ALDH) in tongue squamous cell carcinoma Tca8113 cell line and observe the biological characteristics of tumor cells expressing high level of ALDH (ALDH(hig)).
The expression of ALDH in tongue squamous cell carcinoma Tca8113 cell line was detected by the flow cytometry. The ALDH(hig) and ALDH(low) subpopulation cells were isolated and cultured in vitro respectively. The ability of differentiation, proliferation and tumorsphere formation were detected. Suppression subtractive hybridization (SSH) was employed to screen the genes overexpressed in ALDH(hig) subpopulation cells.
The ALDH(hig) cells in tongue squamous cell carcinoma Tca8113 cell line was rarely 1.3%. Compared with ALDH(low) cells and unsorted cells, ALDH(hig) cells demonstrated increased proliferation capacity. After culture in vitro, the percentage of ALDH(hig) cells decreased gradually to the level of that in unsorted tumor cells. The ALDH(hig) subpopulation cells could survive in serum free medium and generate tumorspheres. SSH analysis revealed these tumor cells also expressed stem cell related genes.
There is a rare subpopulation cells expressing ALDH highly, which displays characteristics similar to stem cells. ALDH may be one of markers for cancer stem cells of tongue squamous cell carcinoma Tca8113 cell line.
检测醛脱氢酶（ALDH）在舌鳞状细胞癌Tca8113细胞系中的表达，并观察高表达ALDH（ALDH(hig)）的肿瘤细胞的生物学特性。
采用流式细胞术检测舌鳞状细胞癌Tca8113细胞系中ALDH的表达。分别分离培养ALDH(hig)和ALDH(low)亚群细胞，检测其分化、增殖及肿瘤球形成能力。采用抑制性消减杂交（SSH）技术筛选在ALDH(hig)亚群细胞中高表达的基因。
舌鳞状细胞癌Tca8113细胞系中ALDH(hig)细胞占比极少，为1.3%。与ALDH(low)细胞及未分选细胞相比，ALDH(hig)细胞增殖能力增强。体外培养后，ALDH(hig)细胞百分比逐渐下降至未分选肿瘤细胞水平。ALDH(hig)亚群细胞能在无血清培养基中存活并形成肿瘤球。SSH分析显示这些肿瘤细胞还表达干细胞相关基因。
舌鳞状细胞癌Tca8113细胞系中存在极少的高表达ALDH的亚群细胞，其表现出与干细胞相似的特性。ALDH可能是舌鳞状细胞癌Tca8113细胞系癌干细胞的标志物之一。
