Clements J R, Beitz A J, Emory C R, Frey W H
Department of Veterinary Biology, University of Minnesota, St. Paul.
Alzheimer Dis Assoc Disord. 1990;4(1):35-42. doi: 10.1097/00002093-199040100-00004.
The ganglioside monoclonal antibody A2B5 has previously been used at the light microscopic level to label Alzheimer neurofibrillary tangles (NFTs). Light microscopic analysis, however, could not reveal whether the A2B5 antibody-labeled NFTs or membrane fragments associated with NFTs. Therefore, we used pre-embedding immunohistochemical electron microscopy to examine A2B5 labeling of NFT. We found that the A2B5 antibody does indeed label a NFT antigen associated with the paired helical filament (PHF) structure, while no significant labeling of membranes or membrane fragments was observed. However, no clear periodicity of the immunogold label on the PHF was found.
神经节苷脂单克隆抗体A2B5此前已用于光镜水平，以标记阿尔茨海默病神经原纤维缠结（NFTs）。然而，光镜分析无法揭示A2B5抗体标记的是NFTs还是与NFTs相关的膜碎片。因此，我们采用包埋前免疫组织化学电子显微镜技术来检测A2B5对NFT的标记情况。我们发现A2B5抗体确实标记了一种与双螺旋丝（PHF）结构相关的NFT抗原，而未观察到对膜或膜碎片的明显标记。然而，在PHF上未发现免疫金标记的清晰周期性。
