意大利 2002-2007 年麻疹病毒的分子流行病学研究。

Molecular epidemiology of measles virus in Italy, 2002-2007.

机构信息

Viral Diseases and Attenuated Vaccines Unit National Institute of Health (Istituto Superiore di Sanità, ISS), Rome, Italy.

出版信息

Virol J. 2012 Nov 23;9:284. doi: 10.1186/1743-422X-9-284.

DOI:10.1186/1743-422X-9-284

PMID:23173726

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3568056/

Abstract

BACKGROUND

The European Regional Office of the World Health Organization (WHO/Europe) developed a strategic approach to halt the indigenous transmission of measles in its 53 Member States by 2015. In view of the goal of measles elimination, it is of great importance to assess the circulation of wild-type measles virus (MV). Genetic analysis is indispensable to understand the epidemiology of measles.

METHODS

Urine and saliva samples were collected between May 2002 and December 2007, in order to find the origins and routes of wild type measles virus circulation. RT-PCR was performed on a total of 414 clinical samples of patients from different Italian regions. The results confirmed the genome presence in 199 samples, out of which 179 were sequenced. The sequences were genotyped by comparing the fragment coding for the carboxyl terminus of the nucleoprotein (450 nucleotides) with that one of the WHO reference strains.

RESULTS

From the year 2002 to the year 2007 phylogenetic analysis of measles sequences showed a predominant circulation of the D7 genotype in the Italian territory for the years 2002-2004. This genotype was replaced by D4 and B3 genotypes in the biennium 2006-2007. During the same period C2, A, D5 and D8 genotypes were also detected.

CONCLUSIONS

Genetic characterization of wild-type MV provides a means to study the transmission pathways of the virus, and is an essential component of laboratory-based surveillance. Knowledge of currently circulating measles virus genotype in Italy will help in monitoring the success of the measles elimination programme and will contribute to evaluate the effectiveness of future vaccination campaigns.

摘要

背景

世界卫生组织欧洲区域办事处（世卫组织欧洲区域办事处）制定了一项战略方法，旨在到 2015 年阻止其 53 个成员国的麻疹本土传播。鉴于消除麻疹的目标，评估野生型麻疹病毒（MV）的循环情况非常重要。遗传分析对于了解麻疹的流行病学是必不可少的。

方法

为了寻找野生型麻疹病毒传播的起源和途径，于 2002 年 5 月至 2007 年 12 月间收集了尿液和唾液样本。对来自意大利不同地区的 414 例患者的临床样本进行了 RT-PCR。结果证实 199 例样本中存在基因组，其中 179 例进行了测序。通过将编码核蛋白羧基末端的片段（450 个核苷酸）与世界卫生组织参考株的相应片段进行比较，对序列进行了基因分型。