Magurano Fabio, Baggieri Melissa, Fortuna Claudia, Bella Antonino, Filia Antonietta, Rota Maria Cristina, Benedetti Eleonora, Bucci Paola, Marchi Antonella, Nicoletti Loredana
Department of Infectious, Parasitic and Immune-Mediated Diseases, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy.
National Centre for Epidemiology, Surveillance and Health Promotion (CNESPS), Istituto Superiore di Sanità (ISS), Via Giano della Bella 34, 00162 Rome, Italy.
J Clin Virol. 2015 Mar;64:34-9. doi: 10.1016/j.jcv.2014.12.019. Epub 2014 Dec 31.
The European Regional Office of the World Health Organization developed a strategic approach to halt the indigenous transmission of measles in its 53 Member States by 2015, World Health Organization [1]. Many European countries, including Italy began the implementation of national programs to reach this goal.
To describe and discuss the results of laboratory activity in measles surveillance, performed from January 2011 to December 2013 by the Italian National Reference Laboratory for Measles and Rubella.
Samples of suspected measles cases were collected from different Italian regions to confirm clinical diagnosis. Anti-measles IgM antibodies detection by Enzyme-Linked Immunosorbent Assay and/or molecular detection by Reverse Transcriptase-Polymerase Chain Reaction assay were performed. Positive samples were sequenced for viral characterization.
According to results from the National Reference Laboratory's activity urine and blood seem to be the best specimens for measles laboratory surveillance. Phylogenetic analysis revealed a co-circulation of the genotypes D4 and D8 during the reviewed period, a cluster of B3 and sporadic cases of D9 and H1.
世界卫生组织欧洲区域办事处制定了一项战略方针，旨在到2015年在其53个成员国中停止麻疹的本土传播，世界卫生组织[1]。包括意大利在内的许多欧洲国家开始实施国家计划以实现这一目标。
描述并讨论2011年1月至2013年12月期间意大利国家麻疹和风疹参考实验室在麻疹监测中开展的实验室活动结果。
从意大利不同地区收集疑似麻疹病例的样本以确诊临床诊断。通过酶联免疫吸附测定法检测抗麻疹IgM抗体和/或通过逆转录聚合酶链反应测定法进行分子检测。对阳性样本进行测序以进行病毒特征分析。
根据国家参考实验室的活动结果，尿液和血液似乎是麻疹实验室监测的最佳样本。系统发育分析显示，在审查期间，基因型D4和D8共同流行，存在B3集群以及D9和H1的散发病例。
