Dionne C A, Kaplan R, Seuánez H, O'Brien S J, Jaye M
Rorer Biotechnology, Inc., King of Prussia, PA 19406.
Biotechniques. 1990 Feb;8(2):190-4.
We describe a new chromosomal assignment method based on the polymerase chain reaction mediated amplification of target sequences in DNAs from somatic cell hybrids. The new method is faster, much more sensitive and less labor intensive than the standard method of chromosome assignment by Southern hybridization analysis of somatic cell hybrid DNAs. The feasibility of the new approach was demonstrated by verifying the assignment of the previously mapped acidic fibroblast growth factor gene to human chromosome 5. The method was employed to assign the related oncogene, FGF-5, to human chromosome 4.
我们描述了一种基于聚合酶链反应介导的体细胞杂种DNA中靶序列扩增的新染色体定位方法。与通过体细胞杂种DNA的Southern杂交分析进行染色体定位的标准方法相比，新方法更快、更灵敏且劳动强度更低。通过验证先前定位的酸性成纤维细胞生长因子基因定位于人类染色体5，证明了新方法的可行性。该方法被用于将相关癌基因FGF-5定位于人类染色体4。
